Communication Dans Un Congrès Année : 2025

Cytokines in naturally occurring bovine respiratory diseases in young cattle at fattening farms using a 15-plex immunoassay

Résumé

Bovine respiratory diseases (BRD) involving several viral and bacterial pathogens are a major burden to the cattle industry. Affected cattle often require antibiotic treatment at fattening. This study aimed to investigate blood cytokine levels in well-characterized cases of BRD. A case-control study was conducted in 2023 in nine fattening operations in Western France. Animals were clinically assessed and underwent thoracic ultrasonography (TUS) weekly during their first month on feed. Animals were classified as sick if they had a rectal temperature ≥ 39.7°C and at least one visual sign of BRD. Farmers decided whether or not to initiate antibiotic treatment that could require one or, in some cases, multiple successive administrations. Blood samples were collected to measure plasma concentration of fifteen innate and adaptive cytokines and chemokines using a commercial bead-based Luminex® immunoassay. Clinically healthy penmates were sampled on the same day. BRD pathogens (Mannheimia haemolytica, Pasteurella multocida, Mycoplasmopsis bovis, Histophilus somni, Bovine Respiratory Syncytial Virus, Bovine Parainfluenza Virus type 3, Bovine Coronavirus, Influenza D Virus) were screened by qPCR on nasal swabs in sick animals and by serology in all animals. Cytokine distributions were analyzed using the Wilcoxon-Mann-Whitney test (two groups) or the Kruskal-Wallis test (multiple groups). The study included 188 young bulls (98 cases, 90 controls), predominantly of Charolais (81.4%) and Limousine (10.7%) breeds, with an average weight of 321(±161) kg and an average age of 9(±5) months. Cases and controls showed no significant differences in breed, weight or age. No cytokines significantly differentiated cases from controls based on clinical definitions alone, but some were associated with pathogen findings. Among cases, IL1-RA levels were higher in animals with consolidations on TUS, and IFN-γ levels were higher in animals requiring multiple antibiotic administrations compared to those untreated or treated once. Individually, cytokines did not differentiate between clinically sick and healthy animals. However, some cytokines demonstrated potential for prognostic applications. Further studies integrating cytokines with other markers are needed to improve BRD diagnosis and prognosis.

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Dates et versions

hal-05108201 , version 1 (11-06-2025)

Identifiants

  • HAL Id : hal-05108201 , version 1

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Maud Rouault, Sébastien Assié, Francois Meurens, Foucras G.. Cytokines in naturally occurring bovine respiratory diseases in young cattle at fattening farms using a 15-plex immunoassay. 2. congrès du Club d'Immunologie et de Vaccinologie Vétérinaire (CIVVET), Club d'Immunologie et de Vaccinologie Vétérinaire (CIVVET); Oniris; INRAE, Apr 2025, Nantes, France. ⟨hal-05108201⟩
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