Poster De Conférence Année : 2025

The Imaging Crystallophore, from protein crystals imaging to in vivo crystallization

Résumé

Determining the 3D structure of proteins is a crucial step for understanding their biological functions and plays a key role in drug and vaccine design in the pharmaceutical industry [1]. X-Ray crystallography (XRD) remains the technique of choice to achieve accurate structural determination of protein crystal, but despite the recent XRD improvements, only about 15% of isolated proteins reach a complete structure determination, due to three major chokepoints: protein purification, crystal nucleation and phase problem [2]. Therefore, our goal is to tackle simultaneously these bottlenecks by providing direct in vivo crystallization. In the past years, our group discovered the “Crystallophore’’, a lanthanide complex capable of inducing new crystallization conditions and improving crystal diffraction quality for numerous proteins, in addition to its known f-element phasing ability [3]. Recently, taking advantage of the luminescence properties of Ln3+ complexes, we developed a new family of ‘’Imaging Crystallophore’’, complexes decorated with specific antennas that provides an easy protein crystal detection exploitable by fluorescent microscopy (one/two photon absorption). Beside the lower nucleating ability of Im-Xo4 than that of the classical Xo4, we demonstrated that a formulation called Mix-Xo4 composed of ImXo4/Xo4 exhibits a synergy between the imaging properties of the former and the nucleating properties of the latter [4]. In our case, the idea is to use recombinant bacteria Escherichia Coli (E. Coli) as biological host, inducing the selected protein overexpression and the consequent crystallization in presence of the Crystallophore species to obtain in vivo protein crystal, directly ready for next XRD step.

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Dates et versions

hal-05097425 , version 1 (04-06-2025)

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  • HAL Id : hal-05097425 , version 1

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Olivier Maury, Leonardo Papa, Amandine Roux, Sigolène Lecuyer. The Imaging Crystallophore, from protein crystals imaging to in vivo crystallization. journée de la SCF-Rhone Alpes, Jun 2025, LYON, France. ⟨hal-05097425⟩
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