Poster De Conférence Année : 2025

Effects of Graphene Oxide on Xenopus Laevis After Multi-Pathway Exposure: Focus on Host Associated Microbiomes

Résumé

Graphene-based nanomaterials, such as graphene oxide (GO), possess unique properties that have spurred their application in industrial and medical fields. However, GO is likely to be released into aquatic ecosystems during its life cycle, necessitating a thorough evaluation of its ecotoxicological risks to ensure its safe use. Amphibians are particularly sensitive to environmental contaminants, and previous studies have demonstrated various toxic effects of GO, including genotoxicity, primarily in larval stages through direct exposure. This study extends the scope by investigating the combined effects of direct and trophic GO exposure on post-metamorphic Xenopus laevis, with a focus on its impact on microbiomes critical for host homeostasis, nutrient absorption, and immunity. To simulate realistic exposure scenarios, dipteran larvae were exposed to different concentrations of GO for 24 hours before being introduced as prey to juvenile Xenopus. These amphibians ingested the GO-laden larvae while also being exposed to GO in the water column. Genomic DNA was extracted from the entire intestine and a skin fragment of Xenopus after 24 and 96 hours to analyze bacterial community composition. The microbiota of the dipteran larvae was also characterized. The results revealed GO-induced shifts in the abundance of key intestinal phyla, such as Firmicutes and Bacteroidetes, which are often associated with dysbiosis. On the skin, significant alterations in microbiome composition were observed, including changes in Proteobacteria, a phylum crucial for resistance to pathogens like Batrachochytrium dendrobatidis. Additionally, the microbiota of dipteran larvae, representing the lower trophic level, was affected by GO exposure. This study highlights the potential ecological consequences of GO contamination at environmentally relevant concentrations, demonstrating its disruptive effects on microbiomes under realistic exposure conditions. The findings underscore the importance of integrating microbiome analyses into ecotoxicological studies to account for indirect effects that may have significant ecological implications.

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hal-05077443 , version 1 (21-05-2025)

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  • HAL Id : hal-05077443 , version 1

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Florian Chapeau, Thomas Moura, van Xuan Nguyen, Marion Vivant, Maialen Barret, et al.. Effects of Graphene Oxide on Xenopus Laevis After Multi-Pathway Exposure: Focus on Host Associated Microbiomes. SETAC Europe 35th Annual Meeting, May 2025, Vienne, Austria. ⟨hal-05077443⟩
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