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              <p>&lt;b&gt;Background:&lt;/b&gt; Mycobacterium abscessus (Mabs) is an environmental bacterium that causes lung infections, especially in patients with cystic fibrosis. Moreover, Mabs exhibits resistance to many antibiotics including anti-tuberculous drugs. Treatment consists of prolonged courses of antibiotic combinations including different molecules. However, its antibiotic resistance coupled with the absence of treatment guidelines leads to high treatment failure. This necessitates the refinement of existing combination therapies to improve patient treatment. This study evaluates the in vitro efficacy of new three-antibiotics combinations from six molecules frequently used in the treatment of Mabs infections: amikacin (AMK), azithromycin (AZM), bedaquiline (BDQ), imipenem (IMI), linezolid (LZD), tigecycline (TIG).&lt;br /&gt;&lt;b&gt;Methods:&lt;/b&gt;&lt;/b&gt; All experiments were performed on the reference strain (ATCC 19977) in 7H9 broth medium at 37 °C and read after 3 days. Based on the efficacy of two-antibiotics combination, efficacy of eleven three-antibiotics including AZM or AMK was studied. 8 concentrations were tested per antibiotic for all three-antibiotics combinations (8*8*8 triplet of concentrations per combination). These combinations were evaluated by calcuting FICimin and by isobolographic analysis.&lt;br /&gt;&lt;b&gt;Results:&lt;/b&gt; All three-antibiotics combinations tested were synergistic with a FICimin&amp;lt;0,75 as defined previously (Bhusal et al., 2005). Among the three-antibiotics combinations tested, 3-D isobolographic analysis revealed that AZM-LZD combined with either BDQ, IMI or TIG have shown the greatest synergies, as these combinations had the most data points below the 0.5 isobole (Figure 1). The other combinations showed only few or no data point below the 0.5 isobole.&lt;br /&gt;&lt;b&gt;Conclusions&lt;/b&gt; Comparing the activity of three-antibiotics showed that among the six molecules tested in combination, the three-antibiotics combinations containing AZM-LZD with either BDQ, IMI or TIG were promising and will be further investigated.&lt;/b&gt;</p>
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