Article Dans Une Revue Veterinary Research Année : 2025

A serologic marker attenuated live vaccine protects piglets against highly pathogenic porcine reproductive and respiratory syndrome virus infection

Jiakai Zhao
  • Fonction : Auteur
Hong Duan
  • Fonction : Auteur
Xu Chen
  • Fonction : Auteur
Binbin Ren
  • Fonction : Auteur
Qianyi Zhu
  • Fonction : Auteur
Pinpin Ji
  • Fonction : Auteur
Yueting Chang
  • Fonction : Auteur
Yani Sun
  • Fonction : Auteur correspondant
  • PersonId : 1536449

Connectez-vous pour contacter l'auteur
Qin Zhao
Connectez-vous pour contacter l'auteur

Résumé

AbstractCurrently, there are no commercial serologic marker or differentiation of infected and vaccinated animal (DIVA) vaccines for the eradication of porcine reproductive and respiratory syndrome virus (PRRSV) infection from pig farms. In a previous study, a nanobody-based competitive ELISA (cELISA) was specifically developed to detect anti-genotype 2 PRRSV (PRRSV-2) antibodies. On the basis of the epitope recognized by the nanobody and the prevalence of PRRSV-2 infection in China, a DIVA vaccine candidate strain was designed and evaluated in the present study. First, an infectious cDNA clone based on the genomic sequence of the highly pathogenic PRRSV-2 (HP-PRRSV) isolate SX-HD was constructed and named rSX-HD. Using the infectious clone as the backbone, a chimeric infectious cDNA clone in which the gene encoding the nucleocapsid (N) protein was replaced with the gene encoding the genotype 1 PRRSV N protein was generated and named rSX-HD2M1. The chimeric PRRSV rSX-HD2M1 was subsequently rescued successfully in Marc-145 cells, which were then passaged for 120 generations for attenuation. A safety study indicated that rSX-HD2M1-F120 is not pathogenic to piglets. In vivo inoculation and challenge experiments suggested that rSX-HD2M1-F120 vaccination significantly reduced serum viral loads and lung tissue lesions and that vaccinated piglets did not show any clinical symptoms or histopathological changes. Furthermore, this recombinant marker virus, in conjunction with the previously developed nanobody-based cELISA, enables serological differentiation between marker virus-infected animals and those infected with wild-type PRRSV-2. These results suggest that rSX-HD2M1-F120 is a good candidate for providing a live attenuated DIVA vaccine against PRRSV-2 infection in piglets.

Fichier principal
Vignette du fichier
13567_2025_Article_1526.pdf (6.72 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence

Dates et versions

hal-05048498 , version 1 (28-04-2025)

Licence

Identifiants

Citer

Jiakai Zhao, Hong Duan, Xu Chen, Binbin Ren, Qianyi Zhu, et al.. A serologic marker attenuated live vaccine protects piglets against highly pathogenic porcine reproductive and respiratory syndrome virus infection. Veterinary Research, 2025, 56 (1), pp.89. ⟨10.1186/s13567-025-01526-8⟩. ⟨hal-05048498⟩

Collections

36 Consultations
110 Téléchargements

Altmetric

Partager

  • More