Communication Dans Un Congrès Année : 2025

Modeling the conformations of biliverdin within a fluorescent protein derived from phytochrome

Résumé

Fluorescent imaging is a technique that allows for the observation of organisms or cells by exploring the phenomenon of fluorescence. The most well-known proteins capable of emitting light is GFP (Green Fluorescent Protein) and has notably been used to trace cancer cells. However, to observe an entire living organism as effectively as possible with such as imaging methods, it is necessary to operate within radiation ranges where the tissue is transparent and absorbs only a small amount of the radiation emitted by the protein. The ideal optical window is located between 600 and 950 nm (near infrared and far-red), which does not correspond to that of GFP. Infrared Fluorescent Proteins (IFP) are fluorescent proteins based on bacterial phytochrome containing a ligand, biliverdin. Biliverdin is a tetrapyrrole covalently bound to one of the protein's cystein. IFPs are precisely optimized to match the ideal optical transparency window of tissues. Molecular modeling proves to be a valuable tool for predicting the most stable structures and the absorption spectrum of the system, as well as understanding the functioning of these systems to improve them. This better understanding will allow proposing mutations of the protein to form new systems that absorb within the optical window.

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Dates et versions

hal-05025159 , version 1 (08-04-2025)

Identifiants

  • HAL Id : hal-05025159 , version 1

Citer

Alicia da Piedade Vieira, Volkan Fındık, Etienne Mangaud, Isabelle Navizet. Modeling the conformations of biliverdin within a fluorescent protein derived from phytochrome. Journée des doctorants du laboratoire MSME 2025, Alicia Da Piedade Vieira; Erwan Le Courric; Hakkoum Abdelhamid, Feb 2025, Champs-sur-Marne, France. ⟨hal-05025159⟩
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