Article Dans Une Revue Transplant International Année : 2024

Antibody Mediated Rejection and T-cell Mediated Rejection Molecular Signatures Using Next-Generation Sequencing in Kidney Transplant Biopsies

1 PARCC (UMR_S 970/ U970) - Paris-Centre de Recherche Cardiovasculaire
2 GenoSplice [Paris]
3 IC UM3 (UMR 8104 / U1016) - Institut Cochin
4 Vall d'Hebron University Hospital [Barcelona]
5 UAB - Universitat Autònoma de Barcelona = Autonomous University of Barcelona = Universidad Autónoma de Barcelona
6 HUG - Hôpitaux Universitaires de Genève = University Hospital of Geneva [Genève]
7 Charité - UniversitätsMedizin = Berlin University Medicine
8 HU Berlin - Humboldt-Universität zu Berlin = Humboldt University of Berlin = Université Humboldt de Berlin
9 BIH - Berlin Institute of Health
10 U1064 Inserm - CR2TI - Centre de Recherche en Transplantation et Immunologie - Center for Research in Transplantation and Translational Immunology
11 Team 4 - U1064 Inserm - CR2TI - Team 4 : Deciphering organ immune regulation in inflammation and transplantation (DORI-t)
12 Team 5 - U1064 Inserm - CR2TI - Team 5 : Neuroinflammation, mechanisms, therapeutic options (NEMO)
13 CIC Plurithématique de Nantes
14 Département de Pathologie [CHU Necker]
15 Service de Néphrologie-transplantation-dialyse [Bordeaux]
16 Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB)
17 Département d'Hépato-Gastroentérologie et de Transplantation Hépatique [CHU Saint-Eloi]
18 Département de Néphrologie et Transplantation d'organes [CHU Toulouse]
19 Infinity - Institut Toulousain des Maladies Infectieuses et Inflammatoires
20 Service de Néphrologie [Lyon]
21 AP-HP - Hôpital Bichat - Claude Bernard [Paris]
22 Service d'Immunologie et d'Histocompatibilité
23 Néphrologie [CHU Bicêtre]
24 Service Néphrologie et transplantation rénale Adultes [CHU Necker]
25 Freie Universität Berlin = Free University of Berlin
26 AP-HP - Hopital Saint-Louis [AP-HP]
Lucie Adoux
Emmanuel Morelon
Renaud Snanoudj

Résumé

Recently, interest in transcriptomic assessment of kidney biopsies has been growing. This study investigates the use of NGS to identify gene expression changes and analyse the pathways involved in rejection. An Illumina bulk RNA sequencing on the polyadenylated RNA of 770 kidney biopsies was conducted. Differentially-expressed genes (DEGs) were determined for AMR and TCMR using DESeq2. Genes were segregated according to their previous descriptions in known panels (microarray or the Banff Human Organ Transplant (B-HOT) panel) to obtain NGS-specific genes. Pathway enrichment analysis was performed using the Reactome and Kyoto Encyclopaedia of Genes and Genomes (KEGG) public repositories. The differential gene expression using NGS analysis identified 6,141 and 8,478 transcripts associated with AMR and TCMR. While most of the genes identified were included in the microarray and the B-HOT panels, NGS analysis identified 603 (9.8%) and 1,186 (14%) new specific genes. Pathways analysis showed that the B-HOT panel was associated with the main immunological processes involved during AMR and TCMR. The microarrays specifically integrated metabolic functions and cell cycle progression processes. Novel NGS-specific based transcripts associated with AMR and TCMR were discovered, which might represent a novel source of targets for drug designing and repurposing.

Fichier principal
Vignette du fichier
ti-37-13043.pdf (3.02 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-04997194 , version 1 (19-03-2025)

Licence

Identifiants

Citer

Esteban Cortes Garcia, Alessia Giarraputo, Maud Racapé, Valentin Goutaudier, Cindy Ursule-Dufait, et al.. Antibody Mediated Rejection and T-cell Mediated Rejection Molecular Signatures Using Next-Generation Sequencing in Kidney Transplant Biopsies. Transplant International, 2024, 37, pp.13043. ⟨10.3389/ti.2024.13043⟩. ⟨hal-04997194⟩
82 Consultations
127 Téléchargements

Altmetric

Partager

  • More