Communication Dans Un Congrès Année : 2024

The plant DCC1 are atypical thioredoxins with a holdase activity

Les DCC1 de plantes sont des thiorédoxines atypiques à activité holdase

Résumé

The thioredoxin superfamily consists of several multigenic families, among which are thioredoxins (TRXs), glutaredoxins (GRXs), protein disulfide isomerases (PDIs). All these proteins share the same structural arrangement, known as thioredoxin fold [1]. They typically use a pair of reactive cysteines in a CXXC motif to perform thiol-disulfide exchange reactions and control the activity or folding of their target proteins. Intriguingly, many proteins possessing one or more CXXC motifs and predicted to adopt a TRX fold remain uncharacterized. Some TRX-like proteins, present in all three domains of life have been referred to as DCC proteins, because of the presence of a DXXCXXC signature at a position similar to the redox active cysteines in TRXs [2]. However, the proteins are extended at both the N-and C-terminal ends. Two to three members are usually found in photosynthetic organisms. In order to biochemically and structurally characterize DCC1 from Arabidopsis thaliana and Populus trichocarpa, the sequences encoding the mature form of the proteins were expressed as recombinant protein in E. coli. Analysis of the redox properties of various full-length and truncated versions, including activity profiling, susceptibility to oxidative modifications and interactions with other oxidoreductases, indicated that although the cysteines are reactive, the proteins are unlikely to be part of the general redox network. Instead, they appeared to form large oligomers with holdase activity. This adds a new layer to the redox chaperone systems, e.g. other TRX or GRX that have been proposed to have such activity.

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Dates et versions

hal-04991152 , version 1 (14-03-2025)

Identifiants

  • HAL Id : hal-04991152 , version 1

Citer

Natacha Donnay, Tiphaine Dhalleine, Flavien Zannini, N. Rouhier, Linda de Bont. The plant DCC1 are atypical thioredoxins with a holdase activity. Plant and Human Sulfur Biology Meeting, Nicolas Rouhier; Jérémy Couturier, Sep 2024, Pont-à-Mousson, France. ⟨hal-04991152⟩
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