<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04985457</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-15T22:40:06+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Study of quinone exchanges in the gut microbiota</title>
            <author role="aut">
              <persName>
                <forename type="first">Morgane</forename>
                <surname>Roger-Margueritat</surname>
              </persName>
              <idno type="halauthorid">2903458-0</idno>
              <affiliation ref="#struct-1069752"/>
              <affiliation ref="#struct-1043049"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sophie-Carole</forename>
                <surname>Chobert</surname>
              </persName>
              <idno type="halauthorid">2932880-0</idno>
              <affiliation ref="#struct-1069752"/>
              <affiliation ref="#struct-1043049"/>
            </author>
            <author role="ctb">
              <persName>
                <forename type="first">Audrey</forename>
                <surname>Le Gouellec</surname>
              </persName>
              <idno type="halauthorid">17873-0</idno>
              <affiliation ref="#struct-1069752"/>
              <affiliation ref="#struct-1043049"/>
            </author>
            <author role="co_last_author">
              <persName>
                <forename type="first">Sophie</forename>
                <surname>Abby</surname>
              </persName>
              <idno type="halauthorid">2779204-0</idno>
              <affiliation ref="#struct-1069752"/>
              <affiliation ref="#struct-1043049"/>
            </author>
            <author role="co_last_author">
              <persName>
                <forename type="first">Fabien</forename>
                <surname>Pierrel</surname>
              </persName>
              <idno type="halauthorid">31805-0</idno>
              <affiliation ref="#struct-1069752"/>
              <affiliation ref="#struct-1043049"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Morgane</forename>
                <surname>Roger-Margueritat</surname>
              </persName>
              <email type="md5">72bf7e33e9bcf272e4186cdf4eb45f11</email>
              <email type="domain">univ-grenoble-alpes.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2025-03-10 21:19:48</date>
              <date type="whenModified">2025-09-27 19:42:15</date>
              <date type="whenReleased">2025-03-10 21:19:48</date>
              <date type="whenProduced">2024-10-07</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="1344156">
                <persName>
                  <forename>Morgane</forename>
                  <surname>Roger-Margueritat</surname>
                </persName>
                <email type="md5">72bf7e33e9bcf272e4186cdf4eb45f11</email>
                <email type="domain">univ-grenoble-alpes.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04985457</idno>
            <idno type="halUri">https://hal.science/hal-04985457</idno>
            <idno type="halBibtex">rogermargueritat:hal-04985457</idno>
            <idno type="halRefHtml">&lt;i&gt;19ème congrès de la Société Française de Microbiologie (SFM)&lt;/i&gt;, Oct 2024, Lille, France</idno>
            <idno type="halRef">19ème congrès de la Société Française de Microbiologie (SFM), Oct 2024, Lille, France</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="IMAG">IMAG</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INPG">Institut polytechnique de Grenoble</idno>
            <idno type="stamp" n="TIMC-IMAG">TIMC</idno>
            <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno>
            <idno type="stamp" n="UGA-EPE">Université Grenoble Alpes [2020-*]</idno>
            <idno type="stamp" n="TIMC-TREE" corresp="TIMC-IMAG">TrEE : Translational microbial Evolution and Engineering</idno>
            <idno type="stamp" n="TEST-UGA">TEST-UGA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="invited" n="0">No</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
            <note type="proceedings" n="0">No</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Study of quinone exchanges in the gut microbiota</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Morgane</forename>
                    <surname>Roger-Margueritat</surname>
                  </persName>
                  <idno type="halauthorid">2903458-0</idno>
                  <affiliation ref="#struct-1069752"/>
                  <affiliation ref="#struct-1043049"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sophie-Carole</forename>
                    <surname>Chobert</surname>
                  </persName>
                  <idno type="halauthorid">2932880-0</idno>
                  <affiliation ref="#struct-1069752"/>
                  <affiliation ref="#struct-1043049"/>
                </author>
                <author role="ctb">
                  <persName>
                    <forename type="first">Audrey</forename>
                    <surname>Le Gouellec</surname>
                  </persName>
                  <idno type="halauthorid">17873-0</idno>
                  <affiliation ref="#struct-1069752"/>
                  <affiliation ref="#struct-1043049"/>
                </author>
                <author role="co_last_author">
                  <persName>
                    <forename type="first">Sophie</forename>
                    <surname>Abby</surname>
                  </persName>
                  <idno type="halauthorid">2779204-0</idno>
                  <affiliation ref="#struct-1069752"/>
                  <affiliation ref="#struct-1043049"/>
                </author>
                <author role="co_last_author">
                  <persName>
                    <forename type="first">Fabien</forename>
                    <surname>Pierrel</surname>
                  </persName>
                  <idno type="halauthorid">31805-0</idno>
                  <affiliation ref="#struct-1069752"/>
                  <affiliation ref="#struct-1043049"/>
                </author>
              </analytic>
              <monogr>
                <meeting>
                  <title>19ème congrès de la Société Française de Microbiologie (SFM)</title>
                  <date type="start">2024-10-07</date>
                  <date type="end">2024-10-09</date>
                  <settlement>Lille</settlement>
                  <country key="FR">France</country>
                </meeting>
                <imprint/>
              </monogr>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">gut microbiota interactions, quinones, cross-feeding, comparative genomics</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="COMM">Conference papers</classCode>
              <classCode scheme="halOldTypology" n="COMM">Conference papers</classCode>
              <classCode scheme="halTreeTypology" n="COMM">Conference papers</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Introduction and ObjectivesThe integrity of the gut microbiota is directly related to host health, and one way to maintain the diversity of the gut microbiota is to promote the exchange of compounds between donor and recipient bacteria, a phenomenon called cross-feeding. In this way, the discovery of new compounds that are exchanged could help keep us healthy. Quinones are key molecules in the energy-producing bacterial respiratory chains and have been proposed to be cross-fed. The aim of this study was to test whether quinone cross-feeding actually exists and to assess its prevalence in the human gut microbiota.Material and MethodsMenaquinone (MK) is the predominant quinone in bacteria, and we performed a bioinformatic annotation with HMM profiles of its biosynthetic pathway in more than 1500 bacterial species from the Unified Human Gastrointestinal Genome (UHGG) datase. Phylogenetic trees with maximum likelihood were obtained to predict transmissions of MK pathways previously detected. Biochemical analysis of MK precursors and potential MK donor species revealed quinone synthesis in recipient bacteria. Heterologous complementation experiments confirmed the functionality of partial MK pathways found in recipient species. Finally, we developed a novel method based on high performance liquid chromatography-mass spectrometry (HPLC-MS) to quantify the diversity of quinones present in gut microbiota samples.Results, Discussion and ConclusionBioinformatic annotations revealed partial MK pathways in bacterial species belonging to all major phyla present in the gut microbiota. The functionality of the genes constituting the partial MK pathways in recipient bacteria was validated experimentally, and cross-feeding of quinone precursors was confirmed. Our phylogenetic analysis revealed that the first part of the MK biosynthesis pathway was repeatedly lost in several bacterial phyla. Overall, our data show that the biosynthesis of quinones in several species depends on cross-feeding from other bacteria, or also possibly from the host. This suggests that this phenomenon is likely to contribute to the equilibrium of the gut microbiota, considering the physiological importance of quinones in bacterial bioenergetics. Our quinone quantification method will now allow us to confirm quinone cross-feeding in vivo, and to evaluate the quinone content in dysbiotic gut microbiota.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="researchteam" xml:id="struct-1069752" status="VALID">
          <orgName>Translational microbial Evolution and Engineering</orgName>
          <orgName type="acronym">TIMC-TrEE</orgName>
          <date type="start">2021-01-01</date>
          <desc>
            <address>
              <addrLine>Faculté de médecine de Grenoble, bâtiment Jean Roget, 38706 La Tronche</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.timc.fr/TrEE</ref>
          </desc>
          <listRelation>
            <relation active="#struct-1043049" type="direct"/>
            <relation active="#struct-301767" type="indirect"/>
            <relation name="UMR5525" active="#struct-441569" type="indirect"/>
            <relation active="#struct-1042703" type="indirect"/>
            <relation active="#struct-1043329" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1043049" status="VALID">
          <idno type="IdRef">14728936X</idno>
          <idno type="ISNI">0000 0004 4687 1979</idno>
          <idno type="RNSR">199511969L</idno>
          <idno type="ROR">https://ror.org/03985kf35</idno>
          <orgName>Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525</orgName>
          <orgName type="acronym">TIMC</orgName>
          <date type="start">2021-01-01</date>
          <desc>
            <address>
              <addrLine>Domaine de la Merci, 38706 La Tronche, France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.timc.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301767" type="direct"/>
            <relation name="UMR5525" active="#struct-441569" type="direct"/>
            <relation active="#struct-1042703" type="direct"/>
            <relation active="#struct-1043329" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-301767" status="VALID">
          <idno type="ROR">https://ror.org/01c7wz417</idno>
          <orgName>VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement</orgName>
          <orgName type="acronym">VAS</orgName>
          <date type="start">2010-01-01</date>
          <desc>
            <address>
              <addrLine>Université de Lyon, VetAgro Sup, 69280 Marcy l'Etoile (campus vétérinaire); Université de Clermont, VetAgro Sup, 63370 Lempdes (campus agronomique)</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.vetagro-sup.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-1042703" status="VALID">
          <idno type="IdRef">240648315</idno>
          <idno type="ROR">https://ror.org/02rx3b187</idno>
          <orgName>Université Grenoble Alpes</orgName>
          <orgName type="acronym">UGA</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>Adresse CS 40700 - 38058 Grenoble cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-grenoble-alpes.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-1043329" status="VALID">
          <idno type="IdRef">026388804</idno>
          <idno type="ROR">https://ror.org/05sbt2524</idno>
          <orgName>Institut polytechnique de Grenoble - Grenoble Institute of Technology</orgName>
          <orgName type="acronym">Grenoble INP</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>46 avenue Félix Viallet 38031 Grenoble Cedex 1</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.grenoble-inp.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-1042703" type="direct"/>
          </listRelation>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>