Article Dans Une Revue Journal of Colloid and Interface Science Année : 2021

Supramolecular tripeptide self-assembly initiated at the surface of coacervates by polyelectrolyte exchange

Miryam Criado-Gonzalez
Deborah Wagner
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Muhammad Haseeb Iqbal
Aymeric Ontani
Alain Carvalho
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Marc Schmutz
Pierre Schaaf
Loïc Jierry
Fouzia Boulmedais

Résumé

Spatial control of supramolecular self-assembly can yield compartmentalized structures, a key feature for the design of artificial cells. Inducing self-assembly from and on compartments is still a challenge. Polyelectrolyte complex coacervates are simple model droplet systems able to reproduce the basic features of membrane-less organelles, appearing in cells. Here, we demonstrate the supramolecular self-assembly of a phosphorylated tripeptide, Fmoc-FFpY (Fmoc: fluorenyl-methoxycarbonyl; F: phenyl alanine, pY: phosphorylated tyrosine), on the surface of poly(l-glutamic acid)/poly(allylamine hydrochloride) (PGA/PAH) complex coacervate microdroplets. The phosphorylated peptides self-assemble, without dephosphorylation, through ion pairing between the phosphate groups of Fmoc-FFpY and the amine groups of PAH. This process provides spontaneous capsules formed by an amorphous polyelectrolyte complex core surrounded by a structured peptide/PAH shell. Similar fibrillar Fmoc-FFpY self-assembled structures are obtained at the interface between the peptide solution and a PGA/PAH polyelectrolyte multilayer, a complex coacervate in the thin film or "multilayer" format. In contact with the peptide solution, PAH chains diffuse out of the coacervate or multilayer film and complex with Fmoc-FFpY at the solution interface, exchanging any PGA with which they were associated. Self-assembly of Fmoc-FFpY, now concentrated by complexation with PAH, follows quickly.

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hal-03110619 , version 1 (14-01-2021)

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Miryam Criado-Gonzalez, Deborah Wagner, Muhammad Haseeb Iqbal, Aymeric Ontani, Alain Carvalho, et al.. Supramolecular tripeptide self-assembly initiated at the surface of coacervates by polyelectrolyte exchange. Journal of Colloid and Interface Science, In press, 588, pp.580-588. ⟨10.1016/j.jcis.2020.12.066⟩. ⟨hal-03110619⟩
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