Article Dans Une Revue Environmental Toxicology and Pharmacology Année : 2025

Chlordecone reduces hyperpolarization-activated-current (Ih) conductance in honeybee

Résumé

The pacemaker channel (HCN) is responsible for electrical activity in a wide range of excitable cells, including those of invertebrates. Using Xenopus oocytes and HEK cell, we show here that HCN-channel from Apis mellifera is activated by hyperpolarization, modulated by cAMP, and blocked by cesium. Its PNa/PK relative permeability is 1:3, and its unitary conductance is 1.5 pS, which is similar to that of the mammalian HCN2 channel. Moreover, bee h-current is blocked by high concentrations of ZD7288, and organochlorine pesticide chlordecone reduces Ih amplitude in a dose-dependent manner (IC50 value was 9.37 µM) and diminishes HCN conductance, while preserving voltage dependence. In contrast, Deltamethrin exhibits no discernible impact. Molecular docking of Chlordecone in a homology model of bee HCN generated by AlphaFold3 suggests a binding site located at the end of the S6 helix that could explain the conductance inhibition caused by Chlordecone.

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hal-04967787 , version 1 (26-02-2025)

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Patrick Bois, Alain Chavanieu, Christophe Magaud, Nassim Fares, Mahira Kaabeche, et al.. Chlordecone reduces hyperpolarization-activated-current (Ih) conductance in honeybee. Environmental Toxicology and Pharmacology, 2025, 114, pp.104659. ⟨10.1016/j.etap.2025.104659⟩. ⟨hal-04967787⟩
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