Article Dans Une Revue Biotechnology and Applied Biochemistry Année : 2003

Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor

Romain Kapel
  • Fonction : Auteur
Naima Nedjar‐arroume
Anne Fertin‐bazus
  • Fonction : Auteur
Pascal Dhulster
Didier Guillochon
  • Fonction : Auteur

Résumé

Peptic hydrolysis of native bovine haemoglobin at pH 3 yields the LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; LVVh-7) opioid peptide corresponding to the residues-31-40 fragment of the β-chain of haemoglobin. This peptide is intermediate in the course of batch hydrolysis and is rapidly degraded. Indeed, it shows an optimum at 3 % degree of hydrolysis (i.e. 2 min of reaction time). The hydrolysis was carried out in a continuous membrane reactor with a space time (ratio of the flux to the reactor volume) set to 2 min (corresponding to optimum LVVh-7 production). This process allows the continuous production of a constant fraction of intermediate peptides containing LVVh-7 for 48 min.

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hal-04946086 , version 1 (20-02-2025)

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Romain Kapel, Renato Froidevaux, Naima Nedjar‐arroume, Anne Fertin‐bazus, Pascal Dhulster, et al.. Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor. Biotechnology and Applied Biochemistry, 2003, 37, pp.317 - 324. ⟨10.1042/ba20020078⟩. ⟨hal-04946086⟩
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