Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV‐haemorphin‐7 (LVVh‐7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor
Résumé
Peptic hydrolysis of native bovine haemoglobin at pH 3 yields the LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe; LVVh-7) opioid peptide corresponding to the residues-31-40 fragment of the β-chain of haemoglobin. This peptide is intermediate in the course of batch hydrolysis and is rapidly degraded. Indeed, it shows an optimum at 3 % degree of hydrolysis (i.e. 2 min of reaction time). The hydrolysis was carried out in a continuous membrane reactor with a space time (ratio of the flux to the reactor volume) set to 2 min (corresponding to optimum LVVh-7 production). This process allows the continuous production of a constant fraction of intermediate peptides containing LVVh-7 for 48 min.
Mots clés
- molecular-mass cut-off
- RP-
- MMCO
- matrix-assisted laser-desorptionionization MS
- MALDI-MS
- LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe)
- LVVh-7
- intermediate peptide
- IP
- degree of hydrolysis
- DH
- regenerated cellulose
- reversed-phase
- molecular-mass cut-off RP-
- matrix-assisted laser-desorptionionization MS MMCO
- LVV-haemorphin-7 (Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe) MALDI-MS
- intermediate peptide LVVh-7
- degree of hydrolysis IP
- regenerated cellulose DH
- ultrafiltration. Abbreviations used: CR
- haemorphins
- decolorization
Domaines
| Origine | Accord explicite pour ce dépôt |
|---|---|
| Licence |