Article Dans Une Revue Development (Cambridge, England) Année : 1997

Hepatocyte growth factor (HGF/SF) is a muscle-derived survival factor for a subpopulation of embryonic motoneurons

Yoichi Yamamoto
  • Fonction : Auteur
Jean Livet
  • Fonction : Auteur
Richard Pollock
  • Fonction : Auteur
Vilma Arce
  • Fonction : Auteur
Odile Delapeyrière
  • Fonction : Auteur
Christopher Henderson
  • Fonction : Auteur

Résumé

ABSTRACT Muscle-derived factors are known to be important for the survival of developing spinal motoneurons, but the molecules involved have not been characterized. Hepatocyte growth factor/scatter factor (HGF/SF) plays an important role in muscle development and motoneuron axon outgrowth. We show that HGF/SF has potent neurotrophic activity (EC50=2 pM) for a subpopulation (40%) of purified embryonic rat motoneurons. Moreover, HGF/SF is an essential component of muscle-derived support for motoneurons, since blocking antibodies to HGF/SF specifically inhibited 65% of the trophic activity of media conditioned by C2/C7 skeletal myotubes, but did not inhibit the trophic activity secreted by Schwann cell lines. High levels of expression of the HGF/SF receptor c-Met in the spinal cord are restricted to subsets of motoneurons, mainly in limb-innervating segments. Consistent with this distribution, cultured motoneurons from limb-innervating brachial and lumbar segments showed a more potent response to HGF/SF than did thoracic motoneurons. By the end of the period of motoneuron cell death, levels of c-Met mRNA in motoneurons were markedly reduced, suggesting that the effects of HGF/SF may be limited to the period of motoneuron cell death. HGF/SF may play an important role during motoneuron development as a muscle-derived survival factor for a subpopulation of limb-innervating motoneurons.

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Dates et versions

hal-04891084 , version 1 (16-01-2025)

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Citer

Yoichi Yamamoto, Jean Livet, Richard Pollock, Alain Garces, Vilma Arce, et al.. Hepatocyte growth factor (HGF/SF) is a muscle-derived survival factor for a subpopulation of embryonic motoneurons. Development (Cambridge, England), 1997, 124 (15), pp.2903-2913. ⟨10.1242/dev.124.15.2903⟩. ⟨hal-04891084⟩
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