Communication Dans Un Congrès Année : 2022

GLUTATHIONE-MEDIATED REDOX REGULATION OF PLANT RESPONSE TO HIGH TEMPERATURE

Résumé

In the context of climate change, global rise of temperature as well as intense heat waves affect plant development and productivity. Among the molecular perturbations that high temperature induce in living cells is the accumulation of reactive oxygen species (ROS), which can damage macromolecules of the cell and perturb the cellular redox state. To cope with deleterious effects of ROS, plant, as other organisms, have developed strategies to scavenge ROS and to regulate their redox state. Among those, glutathione play a major role in maintaining the cellular redox state and the function of key antioxidant enzymes like peroxidases. Here, we investigated the contribution of the redox systems in plant adaptation to high temperature. We studied two different high temperature regimes: a rise of ambient temperature to 27°C inducing a plant developmental adaptation program called thermomorphogenesis, and a 37°C treatment mimicking intense heat wave and affecting plant viability. Using the genetically encoded redox marker roGFP, we show that high temperature regimes lead to cytoplasm and nuclear oxidation and impact profoundly the glutathione pool rather than the glutathione redox state. Moreover, plants are able to restore the glutathione pool within a few hours, which likely contribute to plant adaptation to high temperature. In contrast, conditional glutathione deficient mutants fail to adapt to intense heat waves or to induce thermomorphogenesis, suggesting that glutathione is involved in both of these heat adaptation mechanisms. We also evaluate by RNAseq analyses, how plant change its genome expression signature upon heat stress and identified a marked genome expression deviation in the glutathione deficient mutant which might contribute to their sensitivity to high temperature. Thus, we define glutathione as a major actor in the adaptation of plant to contrasting high temperature regimes.

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Dates et versions

hal-04886953 , version 1 (14-01-2025)

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  • HAL Id : hal-04886953 , version 1

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Avilien Dard, Laetitia Bariat, Alizée Weiss, Nathalie Picault, Frédéric Pontvianne, et al.. GLUTATHIONE-MEDIATED REDOX REGULATION OF PLANT RESPONSE TO HIGH TEMPERATURE. EMBO Workshop "Molecular responses of plants facing climate change", Jun 2022, Montpellier, France. ⟨hal-04886953⟩
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