Cours Année : 2022

Metabarcoding, FROGS training - Oct 2022

Résumé

This training session, in webinar, is designed to help you to deal with NGS data of 16S, 18S, ITS, COI, and other DNAs produced with MiSeq/HiSeq from Illumina technologies in the Galaxy workbench. You will discover how to use a Galaxy instance, run bioinformatics analysis on your data (clean reads, clusterize them, do the taxonomic affiliation, etc.) and perform statistics to interpret your results with the FROGS pipeline. With FROGS you can also make functional inference and hypothesize what metabolic functions are present in a microbial community. Biologists interested in microbiota from various environments (intestinal, soil, aquatic, food, etc.) use genetic markers to know their composition and follow their evolution - metabarcoding. FROGS is a software package for accurate, simple and robust processing of metabarcoding sequencing reads. FROGS uses standard methods and tools combined with original and innovative approaches (such as the support of yeast and fungal markers). FROGS offers in addition to the expected outputs (abundance table of the species in the environment and their affiliations), numerous graphs and statistics allowing biologists an enriched support of their analyses. Its use is open to both non-experts and experts thanks to the launch of the tools via the Galaxy platforms or via the command line. Our objective is to make future FROGS users autonomous in the use of the 28 proposed tools and in the interpretation of the results obtained.

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Metabarcoding, FROGS training – Oct 2022.pdf (60.39 Ko) Télécharger le fichier
A_FROGS_October_2022_Galaxy_practice.pdf (1.79 Mo) Télécharger le fichier
B_FROGS_October_2022_FROGS_basics.pdf (8.38 Mo) Télécharger le fichier
C_FROGS_October_2022_FROGS_Stat.pdf (7.51 Mo) Télécharger le fichier
D_FROGS_October_2022_FROGS_ITS.pdf (5.16 Mo) Télécharger le fichier
E_FROGS_October_2022_FROGS_Func.pdf (2.68 Mo) Télécharger le fichier
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Dates et versions

hal-04885780 , version 1 (14-01-2025)

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Identifiants

  • HAL Id : hal-04885780 , version 1

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Géraldine Pascal, Lucas Auer. Metabarcoding, FROGS training - Oct 2022. Master. France. 2022. ⟨hal-04885780⟩
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