Poster De Conférence Année : 2023

Directionally tuned signals in mouse subicular complex during passive rotation using high-density probes

Résumé

Session PSTR214 - Cross-Modal Processing and Multisensory Integration PSTR214.11 / EE19 - Directionally tuned signals in mouse subicular complex during passive rotation using high-density probes Abstract Head-direction (HD) cells are neurons coding for the animal’s head direction in space. They function as the brain’s compass and are found in several cortical and subcortical areas. The HD system activity is generated based on vestibular signals and controlled by and anchored to visual inputs of external landmark cues. How multisensory signals are processed during passive rotation remains unclear, with the pre- and postsubiculum as a prime candidate for the integration of vestibular based anterior thalamic and visual dominated retrosplenial cortical signals. We examined this question in a novel paradigm where we controlled both vestibular and visual cues. Head-fixed mice were rotated on a motorized stage in a pseudo-random fashion, with peak speeds of 150°/sec and accelerations up to 300°/sec2, covering all angles. Visual cues were projected on a semi-transparent dome surrounding the animal. We investigated neuronal activity of single neurons and populations of neurons in pre-, post-, parasubiculum and subiculum, using Neuropixels probes. The recording protocol was run in the recording room as a control condition, then compared to test conditions where a single visual landmark was projected on the dome that could be shifted by 90° to examine cue control of directionally tuned signals. A darkness condition was used to examine the stability of directional signals in the absence of landmarks. Angular tuning was quantified by dividing the number of spikes by the occupancy per bin, and the Rayleigh vector (R) was calculated for each individual cell. In a first dataset, we recorded 606 well separated units from subicular areas (n=8 mice), of which 61% showed directional tuning (R>0.3, p<0.05). Some units showed mixed selectivity, tuned to head direction and to angular head velocity. Tuning curves of subicular HD cells showed that the HD system is controlled by visual landmarks under the dome, following stripe shifts between conditions. However, no directional tuning was observed during the darkness condition. Nevertheless, using pairwise correlation analysis, we show that the same coherence of activity of HD cells from other conditions is maintained in darkness. These results show that in animals under passive rotation, we are able to record coherently tuned signals from neurons in subicular areas that are under the control of virtual visual cues. Taken together, our work will provide new insights on multisensory processing in the presubiculum and the HD function in head-fixed paradigms.

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Dates et versions

hal-04881587 , version 1 (12-01-2025)

Identifiants

  • HAL Id : hal-04881587 , version 1

Citer

Marin Dauguet, Flavia Aluisi, Mark Wexler, Jean Laurens, Michael Graupner, et al.. Directionally tuned signals in mouse subicular complex during passive rotation using high-density probes. Society for Neuroscience meeting, 2023, Washington, Nov 2023, Washington DC, United States. Session PSTR214 - Cross-Modal Processing and Multisensory Integration PSTR214.11 / EE19. ⟨hal-04881587⟩
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