Poster De Conférence Année : 2024

Deciphering environmental integrons in pristine environment reveals their significance for ecosystem functioning and health

Résumé

Environmental integrons (EI) are genetic elements requiring in-depth study in a One Health perspective, due to their capacity to disseminate resistance genes at risk for human and animals. Yet, contrary to clinical integrons, the eco-evolutionary significance of EI and their role in bacterial adaptation are still elusive. Here, we investigate the diversity and spatio-temporal dynamics of EI in a French peatland to decipher their ecology and evolution in poorly-anthropized ecosystems. Untargeted metagenomic combining short- and long-read sequencing over 24 samples revealed 2875 EI elements including complete integrons, CALINs (gene cassette arrays) and In0 (integron integrase genes). Over the 515 MAGs, 35 harboured complete EI. A wide range of elements were hosted by Acidobacteriota and Nitrospirota, both key phyla in peatland functioning. As well, poorly characterized phyla such as Binatota and Gemmatimonadota exhibited EI while Archaea harboured only CALINs. Moreover, in agreement with previous studies, Alphaproteobacteria and Actinobacteriota recurrently lacked EI. Integron integrase genes distribution evidenced structuring along the environmental gradient. Importantly, no clinical integrons were detected suggesting environmental filtering and/or poor anthropization. However, several gene cassettes being resistance determinants may be at risk. The recovery of integron integrase and gene cassette transcripts suggested that integrase might trigger the dynamics of some elements while certain gene cassettes could play a role in the peatland functioning. Together, our results show that EI do play a role in natural context and may serve as reservoir of resistant genes.

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hal-04838930 , version 1 (15-12-2024)

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  • HAL Id : hal-04838930 , version 1

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Raphaëlle Péguilhan, Eva Sandoval-Quintana, Etienne Richy, Neus Paredes Felipe, Christine Cagnon, et al.. Deciphering environmental integrons in pristine environment reveals their significance for ecosystem functioning and health. Environmental and Agronomical Genomics Symposium, Feb 2024, Toulouse, France. ⟨hal-04838930⟩
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