<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04829910</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-02T05:54:22+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Panel Design and Optimization for High‐Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry</title>
            <author role="aut">
              <persName>
                <forename type="first">Laura</forename>
                <surname>Ferrer‐font</surname>
              </persName>
              <idno type="halauthorid">3334172-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christophe</forename>
                <surname>Pellefigues</surname>
              </persName>
              <idno type="halauthorid">1225142-0</idno>
              <affiliation ref="#struct-60359"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Johannes</forename>
                <forename type="middle">U</forename>
                <surname>Mayer</surname>
              </persName>
              <idno type="halauthorid">2850381-0</idno>
              <affiliation ref="#struct-60359"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sam</forename>
                <forename type="middle">J</forename>
                <surname>Small</surname>
              </persName>
              <idno type="halauthorid">3334173-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Maria</forename>
                <forename type="middle">C</forename>
                <surname>Jaimes</surname>
              </persName>
              <idno type="halauthorid">3334174-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Kylie</forename>
                <forename type="middle">M</forename>
                <surname>Price</surname>
              </persName>
              <email type="md5">cc0dde27ede5f1c081c660edf1c03677</email>
              <email type="domain">malaghan.org.nz</email>
              <idno type="idhal" notation="numeric">1476753</idno>
              <idno type="halauthorid" notation="string">3334175-1476753</idno>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Christophe</forename>
                <surname>Pellefigues</surname>
              </persName>
              <email type="md5">1f6da458042d5e22ddea5b31eb412eea</email>
              <email type="domain">inserm.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2025-01-14 14:40:00</date>
              <date type="whenModified">2025-01-22 15:24:55</date>
              <date type="whenReleased">2025-01-22 15:24:55</date>
              <date type="whenProduced">2020-03</date>
              <date type="whenEndEmbargoed">2025-01-14</date>
              <ref type="file" target="https://hal.science/hal-04829910v1/document">
                <date notBefore="2025-01-14"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-04829910v1/file/Panel%20Design%20and%20Optimization%20Protocol%20for%20High-Dimensional%20Immunophenotyping%20Assays%20using%20Spectral%20Flow%20Cytometry.pdf" id="file-4886222-4246775">
                <date notBefore="2025-01-14"/>
              </ref>
              <ref type="externalLink" target="https://www.biorxiv.org/content/biorxiv/early/2019/09/27/784884.full.pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="1475817">
                <persName>
                  <forename>Christophe</forename>
                  <surname>Pellefigues</surname>
                </persName>
                <email type="md5">1f6da458042d5e22ddea5b31eb412eea</email>
                <email type="domain">inserm.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04829910</idno>
            <idno type="halUri">https://hal.science/hal-04829910</idno>
            <idno type="halBibtex">ferrerfont:hal-04829910</idno>
            <idno type="halRefHtml">&lt;i&gt;Current Protocols in Cytometry&lt;/i&gt;, 2020, 92, &lt;a target="_blank" href="https://dx.doi.org/10.1002/cpcy.70"&gt;&amp;#x27E8;10.1002/cpcy.70&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Current Protocols in Cytometry, 2020, 92, &amp;#x27E8;10.1002/cpcy.70&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-4886222-4246775"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt/>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Panel Design and Optimization for High‐Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Laura</forename>
                    <surname>Ferrer‐font</surname>
                  </persName>
                  <idno type="halauthorid">3334172-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christophe</forename>
                    <surname>Pellefigues</surname>
                  </persName>
                  <idno type="halauthorid">1225142-0</idno>
                  <affiliation ref="#struct-60359"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Johannes</forename>
                    <forename type="middle">U</forename>
                    <surname>Mayer</surname>
                  </persName>
                  <idno type="halauthorid">2850381-0</idno>
                  <affiliation ref="#struct-60359"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sam</forename>
                    <forename type="middle">J</forename>
                    <surname>Small</surname>
                  </persName>
                  <idno type="halauthorid">3334173-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Maria</forename>
                    <forename type="middle">C</forename>
                    <surname>Jaimes</surname>
                  </persName>
                  <idno type="halauthorid">3334174-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Kylie</forename>
                    <forename type="middle">M</forename>
                    <surname>Price</surname>
                  </persName>
                  <email type="md5">cc0dde27ede5f1c081c660edf1c03677</email>
                  <email type="domain">malaghan.org.nz</email>
                  <idno type="idhal" notation="numeric">1476753</idno>
                  <idno type="halauthorid" notation="string">3334175-1476753</idno>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="OLD">135278</idno>
                <idno type="issn">1934-9300</idno>
                <title level="j">Current Protocols in Cytometry</title>
                <imprint>
                  <publisher>Wiley</publisher>
                  <biblScope unit="volume">92</biblScope>
                  <date type="datePub">2020-03</date>
                </imprint>
              </monogr>
              <idno type="biorxiv">784884</idno>
              <idno type="doi">10.1002/cpcy.70</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">fluorescent antibodies r high-dimensional panel design r immunophenotyping r optimization r spectral flow cytometry</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>&lt;div&gt;&lt;p&gt;Technological advances in fluorescence flow cytometry and an ever-expanding understanding of the complexity of the immune system have led to the development of large (20+ parameters) flow cytometry panels. However, as panel complexity and size increase, so does the difficulty involved in designing a highquality panel, accessing the instrumentation capable of accommodating large numbers of parameters, and analyzing such high-dimensional data. A recent advancement is spectral flow cytometry, which in contrast to conventional flow cytometry distinguishes the full emission spectrum of each fluorophore across all lasers, rather than identifying only the peak of emission. Fluorophores with a similar emission maximum but distinct off-peak signatures can therefore be accommodated within the same flow cytometry panel, allowing greater flexibility in terms of panel design and fluorophore detection. Here, we highlight the specific characteristics of spectral flow cytometry and aim to guide users through the process of building, designing, and optimizing high-dimensional spectral flow cytometry panels using a comprehensive step-by-step protocol. Special considerations are also given for using highly overlapping dyes, and a logical selection process for optimal marker-fluorophore assignment is provided.&lt;/p&gt;&lt;/div&gt;</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-60359" status="VALID">
          <orgName>Malaghan Institute of Medical Research [Wellington, New Zealand]</orgName>
          <desc>
            <address>
              <country key="NZ"/>
            </address>
            <ref type="url">https://www.malaghan.org.nz/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>