Lichen substances have been firstly described in the 1870s and most of them have been evaluated for their activity on planktonic microorganisms (bacteria and fungi). More recently, microorganisms colonizing the lichen thallus have been isolated and identified giving access to a wild diversity of culturable microorganisms. The increasing research in lichens associated microbiome in recent years, has emphased a wide range of metabolites as a potential source of bioactive compounds. In parallel, humans are facing microbial resistance to conventional antimicrobial drugs. One of the reasons is the biofilm lifestyle of microorganisms. Indeed, the aggregation of microbial communities inside biofilms is now well‐known and characterized and some possible ways to fight and destroy biofilms are identified (quorum sensing inhibitors,…). The present review aims to summarize the anti‐biofilm potential of lichen metabolites and those from their associated microorganisms (bacteria and/or fungi). Are the metabolites isolated from lichens and their associated fungi displaying any anti‐biofilm activity? This literature synthesis highlights the metabolites of interest as new anti‐biofilm drugs and shows the lack of current biological research dealing with biofilm and lichen metabolites. Only two lichen metabolites, usnic acid and evernic acid, have been evaluated both as antifungal and antibacterial biofilm compounds.