Poster De Conférence Année : 2023

Development of a new detection method for small analytes based on the fluorogenic supramolecular assembly of aptamers

Résumé

There is an increasing demand for the detection of small molecules in order to monitor human health (molecular markers of diseases, toxins, heavy metals, etc.) A notable limitation of aptasensors for small analytes is their low sensitivity due to non-specific and false positives signals. To circumvent this drawback, the split aptamer strategy was developed by Stojanovic et al [1], in which an aptamer is split into two fragments that can specifically form a ternary assembly in the presence of the ligand. Because of this dual recognition mechanism, the resulting specificity and sensitivity is significantly increased. Unfortunately, splitting decreases affinity, and the resulting ternary complex is quite unstable. To overcome this issue, Yang et al [2] introduced a host-guest signaling system into a split-aptasensor in which β-cyclodextrin and dansyl were respectively conjugated at the ends of the two fragments of an adenosine aptamer: the inclusion complex provided a supplementary interaction and induced stabilizing effects. In this project, both the stabilizing interaction and the transduction signal are due to the formation of a fluorescent indolizine occurring upon binding of the split-aptamers to their target, with each strand carrying a precursor of the fluorophore (figure A). In this construct, the [3+2] dipolar cycloaddition of an in situ generated pyridinium ylide with an activated alkyne is said to be DNA- templated as it is catalyzed by the binding of the aptamer strands to their ligand

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Dates et versions

hal-04794962 , version 1 (21-11-2024)

Identifiants

  • HAL Id : hal-04794962 , version 1

Citer

Rim Karkori, Nicolas Spinelli, Isabelle Baussanne. Development of a new detection method for small analytes based on the fluorogenic supramolecular assembly of aptamers. 3ème Journée du GDR ChemBio, Jun 2023, Illkirch, Strasbourg, France. ⟨hal-04794962⟩
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