Article Dans Une Revue Nature Medicine Année : 2020

Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein

Seth Zost
Pavlo Gilchuk
  • Fonction : Auteur
Rachel Nargi
  • Fonction : Auteur
Rachel Sutton
  • Fonction : Auteur
Naveenchandra Suryadevara
  • Fonction : Auteur
Elaine Chen
Elad Binshtein
Swathi Shrihari
Mario Ostrowski
  • Fonction : Auteur
Jonathan Didier
  • Fonction : Auteur
Keith Macrenaris
  • Fonction : Auteur
Taylor Jones
  • Fonction : Auteur
Samuel Day
  • Fonction : Auteur
Luke Myers
F. Eun-Hyung Lee
Doan Nguyen
Ignacio Sanz
David Martinez
  • Fonction : Auteur
Paul Rothlauf
  • Fonction : Auteur
Sean Whelan
Ralph Baric
  • Fonction : Auteur
Larissa Thackray
Michael Diamond
  • Fonction : Auteur
Robert Carnahan
James Crowe

Résumé

Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms.

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Dates et versions

hal-04794185 , version 1 (20-11-2024)

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Citer

Seth Zost, Pavlo Gilchuk, Rita Chen, James Brett Case, Joseph Reidy, et al.. Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein. Nature Medicine, 2020, 26 (9), pp.1422-1427. ⟨10.1038/s41591-020-0998-x⟩. ⟨hal-04794185⟩
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