<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04793055</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-17T01:40:02+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Is a real-time quantifiable liquid biopsy achievable using a microfluidic lab-on-chip ?</title>
            <author role="aut">
              <persName>
                <forename type="first">Veronica</forename>
                <surname>Casali</surname>
              </persName>
              <idno type="halauthorid">3034940-0</idno>
              <affiliation ref="#struct-1043059"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Ingrid</forename>
                <forename type="middle">Clerc</forename>
                <surname>Guithon</surname>
              </persName>
              <idno type="halauthorid">3303911-0</idno>
              <affiliation ref="#struct-1043059"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Boudewijn</forename>
                <forename type="middle">van Der</forename>
                <surname>Sanden</surname>
              </persName>
              <idno type="halauthorid">1092328-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Olivier</forename>
                <surname>Stephan</surname>
              </persName>
              <idno type="halauthorid">204811-0</idno>
              <affiliation ref="#struct-1043294"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Laetitia</forename>
                <surname>Gredy</surname>
              </persName>
              <idno type="halauthorid">2279996-0</idno>
              <affiliation ref="#struct-1043294"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Isabelle</forename>
                <surname>Vilgrain</surname>
              </persName>
              <idno type="halauthorid">402415-0</idno>
              <affiliation ref="#struct-555292"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Donald</forename>
                <forename type="middle">K</forename>
                <surname>Martin</surname>
              </persName>
              <email type="md5">03a679787b6d67ccc16e0d70389cfdd6</email>
              <email type="domain">imag.fr</email>
              <idno type="idhal" notation="string">donald-k-martin</idno>
              <idno type="idhal" notation="numeric">1041503</idno>
              <idno type="halauthorid" notation="string">1090835-1041503</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5913-2372</idno>
              <idno type="IDREF">https://www.idref.fr/160585279</idno>
              <idno type="ISNI">http://isni.org/isni/0000000064595251</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/HSE-9912-2023</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/JOA-2660-2023</idno>
              <idno type="VIAF">https://viaf.org/viaf/94630103</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/HSE-9912-2023</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/JOA-2660-2023</idno>
              <affiliation ref="#struct-1043059"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>ISABELLE</forename>
                <surname>VILGRAIN</surname>
              </persName>
              <email type="md5">88a56f662e40611730863cfee54f8245</email>
              <email type="domain">cea.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2024-11-20 12:37:50</date>
              <date type="whenModified">2025-09-27 19:18:03</date>
              <date type="whenReleased">2024-11-25 10:59:14</date>
              <date type="whenProduced">2023-10-01</date>
              <date type="whenEndEmbargoed">2024-11-20</date>
              <ref type="file" target="https://hal.science/hal-04793055v1/document">
                <date notBefore="2024-11-20"/>
              </ref>
              <ref type="file" subtype="greenPublisher" n="1" target="https://hal.science/hal-04793055v1/file/EurobiotechJv7p189y2023%20%28short%20review%20on%20our%20Uroloc%20LoC%20and%20liquid%20biopsy%29.pdf" id="file-4793055-4181294">
                <date notBefore="2024-11-20"/>
              </ref>
              <ref type="externalLink" target="https://sciendo.com/pdf/10.2478/ebtj-2023-0014"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="696037">
                <persName>
                  <forename>ISABELLE</forename>
                  <surname>VILGRAIN</surname>
                </persName>
                <email type="md5">88a56f662e40611730863cfee54f8245</email>
                <email type="domain">cea.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04793055</idno>
            <idno type="halUri">https://hal.science/hal-04793055</idno>
            <idno type="halBibtex">casali:hal-04793055</idno>
            <idno type="halRefHtml">&lt;i&gt;The EuroBiotech Journal&lt;/i&gt;, 2023, 7 (4), pp.189 - 195. &lt;a target="_blank" href="https://dx.doi.org/10.2478/ebtj-2023-0014"&gt;&amp;#x27E8;10.2478/ebtj-2023-0014&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">The EuroBiotech Journal, 2023, 7 (4), pp.189 - 195. &amp;#x27E8;10.2478/ebtj-2023-0014&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by-nc-nd/4.0/">CC BY-NC-ND 4.0 - Attribution - Non-commercial use - No Derivative Works<ref corresp="#file-4793055-4181294"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="CEA">CEA - Commissariat à l'énergie atomique</idno>
            <idno type="stamp" n="UGA">HAL Grenoble Alpes</idno>
            <idno type="stamp" n="IMAG">IMAG</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INPG">Institut polytechnique de Grenoble</idno>
            <idno type="stamp" n="TIMC-IMAG">TIMC</idno>
            <idno type="stamp" n="TIMC-IMAG-SYNABI" corresp="TIMC-IMAG">SyNaBi : Systèmes Nanobiotechnologiques et Biomimétiques</idno>
            <idno type="stamp" n="LIPHY" corresp="CNRS">Laboratoire interdisciplinaire de Physique</idno>
            <idno type="stamp" n="IRIG" corresp="CEA">Institut de Recherche Interdisciplinaire de Grenoble</idno>
            <idno type="stamp" n="CEA-GRE" corresp="CEA">CEA Grenoble</idno>
            <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno>
            <idno type="stamp" n="UGA-EPE">Université Grenoble Alpes [2020-*]</idno>
            <idno type="stamp" n="FRANCE-GENOMIQUE">France Génomique</idno>
            <idno type="stamp" n="BIOMICS_IP" corresp="FRANCE-GENOMIQUE">Biomics</idno>
            <idno type="stamp" n="TEST-UGA">TEST-UGA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Is a real-time quantifiable liquid biopsy achievable using a microfluidic lab-on-chip ?</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Veronica</forename>
                    <surname>Casali</surname>
                  </persName>
                  <idno type="halauthorid">3034940-0</idno>
                  <affiliation ref="#struct-1043059"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Ingrid</forename>
                    <forename type="middle">Clerc</forename>
                    <surname>Guithon</surname>
                  </persName>
                  <idno type="halauthorid">3303911-0</idno>
                  <affiliation ref="#struct-1043059"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Boudewijn</forename>
                    <forename type="middle">van Der</forename>
                    <surname>Sanden</surname>
                  </persName>
                  <idno type="halauthorid">1092328-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Olivier</forename>
                    <surname>Stephan</surname>
                  </persName>
                  <idno type="halauthorid">204811-0</idno>
                  <affiliation ref="#struct-1043294"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Laetitia</forename>
                    <surname>Gredy</surname>
                  </persName>
                  <idno type="halauthorid">2279996-0</idno>
                  <affiliation ref="#struct-1043294"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Isabelle</forename>
                    <surname>Vilgrain</surname>
                  </persName>
                  <idno type="halauthorid">402415-0</idno>
                  <affiliation ref="#struct-555292"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Donald</forename>
                    <forename type="middle">K</forename>
                    <surname>Martin</surname>
                  </persName>
                  <email type="md5">03a679787b6d67ccc16e0d70389cfdd6</email>
                  <email type="domain">imag.fr</email>
                  <idno type="idhal" notation="string">donald-k-martin</idno>
                  <idno type="idhal" notation="numeric">1041503</idno>
                  <idno type="halauthorid" notation="string">1090835-1041503</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5913-2372</idno>
                  <idno type="IDREF">https://www.idref.fr/160585279</idno>
                  <idno type="ISNI">http://isni.org/isni/0000000064595251</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/HSE-9912-2023</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/JOA-2660-2023</idno>
                  <idno type="VIAF">https://viaf.org/viaf/94630103</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/HSE-9912-2023</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/JOA-2660-2023</idno>
                  <affiliation ref="#struct-1043059"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">133410</idno>
                <idno type="eissn">2564-615X</idno>
                <title level="j">The EuroBiotech Journal</title>
                <imprint>
                  <publisher>Sciendo</publisher>
                  <biblScope unit="volume">7</biblScope>
                  <biblScope unit="issue">4</biblScope>
                  <biblScope unit="pp">189 - 195</biblScope>
                  <date type="datePub">2023-10-01</date>
                </imprint>
              </monogr>
              <idno type="doi">10.2478/ebtj-2023-0014</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">3D microenvironment cell monolayer microfluidic lab-on-chip secretome real-time measurement</term>
                <term xml:lang="en">3D microenvironment</term>
                <term xml:lang="en">cell monolayer</term>
                <term xml:lang="en">microfluidic</term>
                <term xml:lang="en">lab-on-chip</term>
                <term xml:lang="en">secretome</term>
                <term xml:lang="en">real-time measurement</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>&lt;div&gt;&lt;p&gt;An increasingly relevant functional measurement is a liquid biopsy to assist in the diagnosis of cancers. The existing approach for liquid biopsy is to utilize microfluidic chips for the isolation of circulating tumor cells (CTCs) or exosomes or extracellular vesicles (EV) from patient samples, and then for the analysis of the cargo contained inside the CTCs, exosomes or EVs. However, such an analysis does not provide a real-time liquid biopsy, since there is a long delay between the time of sample collection and the results from the analysis. Microfluidic chip-formats also provide the capability to mimic tissue functions from the analysis of small numbers of cells cultured in the chip. Analysis of the secreted molecules from such cells could provide a measurement of the secretome, which could be analogous to a liquid biopsy. A 3D structural organization of cells in microfluidic chips is usually in the form of organoids or spheroids. The analysis of organoids or spheroids is well-adapted for immunohistochemistry or ELISA-type identification of surface markers, but not for real-time analysis of secreted molecules since the fluid and molecules in the interior volume of the organoid or spheroid is not accessible in real-time. We have recently proposed an alternative novel design for a microfluidic chip format comprising 3D micro-niches that provide a real-time analysis of secretions produced directly from small numbers of cells. The microfluidic chip with 3D micro-niches then analyses the secretions from these monolayers in real-time ("secretome"). The microfluidic chip includes electronic biosensors that provide real-time measurement of secreted molecules. This short review concludes with a proposition for the means to utilize this novel microfluidic chip to function as a real-time and quantifiable diagnostic screening device to differentiate cancerous cells from healthy cells.&lt;/p&gt;&lt;/div&gt;</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="researchteam" xml:id="struct-1043059" status="VALID">
          <orgName>Systèmes Nanobiotechnologiques et Biomimétiques</orgName>
          <orgName type="acronym">TIMC-SyNaBi</orgName>
          <date type="start">2021-01-01</date>
          <desc>
            <address>
              <addrLine>Pavillon Taillefer, Rond-point de la Croix de Vie, 38700 La Tronche</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.timc.fr/synabi</ref>
          </desc>
          <listRelation>
            <relation active="#struct-1043049" type="direct"/>
            <relation active="#struct-301767" type="indirect"/>
            <relation name="UMR5525" active="#struct-441569" type="indirect"/>
            <relation active="#struct-1042703" type="indirect"/>
            <relation active="#struct-1043329" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1043294" status="VALID">
          <idno type="IdRef">035460849</idno>
          <idno type="ISNI">0000 0000 9272 9931</idno>
          <idno type="RNSR">201122569N</idno>
          <idno type="ROR">https://ror.org/023n9q531</idno>
          <orgName>Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères]</orgName>
          <orgName type="acronym">LIPhy</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>140 rue de la Physique38058 Grenoble cedex 9</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www-liphy.ujf-grenoble.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR5588" active="#struct-441569" type="direct"/>
            <relation active="#struct-1042703" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-555292" status="VALID">
          <idno type="IdRef">240648846</idno>
          <idno type="RNSR">201923169F</idno>
          <idno type="ROR">https://ror.org/00byxdz40</idno>
          <idno type="Wikidata">Q108737333</idno>
          <orgName>Institut de Recherche Interdisciplinaire de Grenoble</orgName>
          <orgName type="acronym">IRIG</orgName>
          <date type="start">2019-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://hal-cea.archives-ouvertes.fr/IRIG/</ref>
          </desc>
          <listRelation>
            <relation name="DRF/IRIG" active="#struct-465913" type="direct"/>
            <relation active="#struct-300016" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1043049" status="VALID">
          <idno type="IdRef">14728936X</idno>
          <idno type="ISNI">0000 0004 4687 1979</idno>
          <idno type="RNSR">199511969L</idno>
          <idno type="ROR">https://ror.org/03985kf35</idno>
          <orgName>Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525</orgName>
          <orgName type="acronym">TIMC</orgName>
          <date type="start">2021-01-01</date>
          <desc>
            <address>
              <addrLine>Domaine de la Merci, 38706 La Tronche, France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.timc.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301767" type="direct"/>
            <relation name="UMR5525" active="#struct-441569" type="direct"/>
            <relation active="#struct-1042703" type="direct"/>
            <relation active="#struct-1043329" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-301767" status="VALID">
          <idno type="ROR">https://ror.org/01c7wz417</idno>
          <orgName>VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement</orgName>
          <orgName type="acronym">VAS</orgName>
          <date type="start">2010-01-01</date>
          <desc>
            <address>
              <addrLine>Université de Lyon, VetAgro Sup, 69280 Marcy l'Etoile (campus vétérinaire); Université de Clermont, VetAgro Sup, 63370 Lempdes (campus agronomique)</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.vetagro-sup.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-1042703" status="VALID">
          <idno type="IdRef">240648315</idno>
          <idno type="ROR">https://ror.org/02rx3b187</idno>
          <orgName>Université Grenoble Alpes</orgName>
          <orgName type="acronym">UGA</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>Adresse CS 40700 - 38058 Grenoble cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-grenoble-alpes.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-1043329" status="VALID">
          <idno type="IdRef">026388804</idno>
          <idno type="ROR">https://ror.org/05sbt2524</idno>
          <orgName>Institut polytechnique de Grenoble - Grenoble Institute of Technology</orgName>
          <orgName type="acronym">Grenoble INP</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>46 avenue Félix Viallet 38031 Grenoble Cedex 1</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.grenoble-inp.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-1042703" type="direct"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-465913" status="VALID">
          <idno type="IdRef">251118436</idno>
          <idno type="RNSR">201622496M</idno>
          <idno type="ROR">https://ror.org/01yvj5k91</idno>
          <idno type="Wikidata">Q52604525</idno>
          <orgName>Direction de Recherche Fondamentale (CEA)</orgName>
          <orgName type="acronym">DRF (CEA)</orgName>
          <date type="start">2016-01-01</date>
          <desc>
            <address>
              <addrLine>Direction de Recherche FondamentaleFundamental Research DivisionCEA Saclay91191 Gif-sur-Yvette</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://ceasciences.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-300016" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300016" status="VALID">
          <idno type="IdRef">026372061</idno>
          <idno type="ISNI">0000000122998025</idno>
          <idno type="ROR">https://ror.org/00jjx8s55</idno>
          <idno type="Wikidata">Q868550</idno>
          <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
          <orgName type="acronym">CEA</orgName>
          <desc>
            <address>
              <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cea.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>