Real-time impedance-activated dielectrophoretic actuation for reconfigurable manipulation of single flowing particles - Archive ouverte HAL
Article Dans Une Revue Lab on a Chip Année : 2024

Real-time impedance-activated dielectrophoretic actuation for reconfigurable manipulation of single flowing particles

Résumé

This work presents an innovative all-electrical platform for selective single-particle manipulation. The platform combines microfluidic impedance cytometry for label-free particle characterization and dielectrophoresis for contactless multi-way particle separation. The microfluidic chip has a straightforward coplanar electrode layout and no particle pre-focusing mechanism is required. An original online algorithm analyzes the impedance signals of each incoming particle and regulates in real time the dielectrophoretic voltages according to a desired control logic. As a proof-of-concept, three operation modes are demonstrated on a mixture of 8, 10, and 12 μm diameter beads: (i) particle position swapping across the channel axis, irrespective of particle size, (ii) size-based particle separation, irrespective of the particle position, and (iii) sorting of a selected sequence of particles. As a perspective, the versatility of impedance cytometry and dielectrophoresis and the possibility of configurin alternative control logics hold promise for Advanced particle and cell manipulation
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Dates et versions

hal-04790945 , version 1 (19-11-2024)

Identifiants

  • HAL Id : hal-04790945 , version 1

Citer

Alexis Lefevre, Cristian Brandi, Adele de Ninno, Filippo Ruggiero, Enrico Verona, et al.. Real-time impedance-activated dielectrophoretic actuation for reconfigurable manipulation of single flowing particles. Lab on a Chip, 2024, 5 (22), pp.5145 - 5154. ⟨hal-04790945⟩
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