Poster De Conférence Année : 2022

Chemical tools for the identification of Lysosomal OligoSaccharide Transporter (LOST)

Résumé

Glycoconjugates and oligosaccharides derived from pathogens and the extracellular matrix are detected by elements of the innate immune system. , More recently free oligosaccharides generated during protein N-glycosylation (fOS) have also been shown to be proinflammatory (fOSp) after demannosylation and contribute to certain rare inherited inflammatory diseases. A non-vesicular fOS subcellular trafficking pathway in which fOS are transported out of the ER into the cytosol relies on unidentified ER oligosaccharide transport machinery (ERT) and, after trimming by cytoplasmic glycosidases (Engase1p and Man2C1p), a lysosomal fOS transport machinery (LOST) in order to be demannosylated into lysosomes. In order to better define the role of this non-vesicular fOS trafficking pathway in normal and disease states, LOST must be manipulated. Therefore, our objective is to develop chemical tools to facilitate the study, characterization and identification of LOST proteins. In this communication, the design and the synthesis of these oligosaccharidic fluorescent, biotinylated and photoactivatable probes will be described. 1) Misra S., et al Front Immunol. 2015 6:201 2) Franchi I., et al. Nat Immunol. 2012 13:325-32 3) Hasan M., et al. Immunity. 2015 43:463-74. 4) We gratefully acknowledged the financial support of this work by the FRM (DCM 20181039551).

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Dates et versions

hal-04784650 , version 1 (15-11-2024)

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  • HAL Id : hal-04784650 , version 1

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Michaël Bosco, Younès Bouzidi, Haifei Gao, Isabelle Chantret, Sébastien Fort, et al.. Chemical tools for the identification of Lysosomal OligoSaccharide Transporter (LOST). Journées de Chimie Organique de la SFC 2022, Nov 2022, Palaiseau, France. ⟨hal-04784650⟩
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