Article Dans Une Revue Methods in Molecular Biology Année : 2024

Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus

Résumé

Non-coding RNAs, including regulatory RNAs (sRNAs), are instrumental in regulating gene expression in pathogenic bacteria, allowing them to adapt to various stresses encountered in their host environments. Staphylococcus aureus is a well-studied model for RNA-mediated regulation of virulence and pathogenicity, with sRNAs playing significant roles in shaping S. aureus interactions with human and animal hosts. By modulating the translation and/or stability of target mRNAs, sRNAs regulate the synthesis of virulence factors and regulatory proteins required for pathogenesis. Moreover, perturbation of the levels of RNA modifications in two other classes of non-coding RNAs, rRNAs and tRNAs, has been proposed to contribute to stress adaptation. However, the study of how these various factors affect translation regulation has often been restricted to specific genes, using in vivo reporters and/or in vitro translation systems. Genome-wide sequencing approaches offer novel perspectives for studying RNAdependent regulation. In particular, ribosome profiling methods provide a powerful resource for characterizing the overall landscape of translational regulation, contributing to a better understanding of S. aureus physiopathology. Here, we describe protocols that we have adapted to perform ribosome profiling in S. aureus.

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hal-04781575 , version 1 (13-11-2024)

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Maximilian Kohl, Béatrice Chane-Woon-Ming, Roberto Bahena-Ceron, Jose Jaramillo-Ponce, Laura Antoine, et al.. Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus. Methods in Molecular Biology, 2024, Methods in Molecular Biology, 2741, pp.73-100. ⟨10.1007/978-1-0716-3565-0_5⟩. ⟨hal-04781575⟩
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