COMBINING GLOBAL TRACKING WITH STATISTICAL CLASSIFICATION TO ANALYZE ENDOCYTOSIS DYNAMICS USING TIRF MICROSCOPY - Archive ouverte HAL
Communication Dans Un Congrès Année : 2017

COMBINING GLOBAL TRACKING WITH STATISTICAL CLASSIFICATION TO ANALYZE ENDOCYTOSIS DYNAMICS USING TIRF MICROSCOPY

Résumé

In this work we set several mathematical tools to study the role of Dynamin and Endophilin in the Clathrin-mediated endocytosis process. Their different dynamics and co-localizations are observed by using TIRF microscopy. We define in particular a novel tracking method in order to track the Clathrin-coated pits and quantify their co-localization with the proteins involved in the process. Thereafter, we perform a statistical analysis of the lifetimes of Clathrin-coated pits tracks by a suitable mixture model. This allows to define different populations of interest and point out the role of Endophilin and Dynamin in the process.

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Dates et versions

hal-04778865 , version 1 (19-11-2024)
hal-04778865 , version 2 (27-11-2024)

Identifiants

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Thibault Lagache, Giacomo Nardi, Laetitia Bertot, Alexandre Grassart, Nathalie Sauvonnet, et al.. COMBINING GLOBAL TRACKING WITH STATISTICAL CLASSIFICATION TO ANALYZE ENDOCYTOSIS DYNAMICS USING TIRF MICROSCOPY. IEEE International Symposium on Biomedical Imaging, Apr 2017, Melbourne (AUS), France. ⟨10.1109/ISBI.2017.7950706⟩. ⟨hal-04778865v2⟩
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