Direct analysis by ultra-high-resolution mass spectrometry of lipid A and phospholipids from Acinetobacter baumannii cells - Archive ouverte HAL
Article Dans Une Revue Biochimie Année : 2024

Direct analysis by ultra-high-resolution mass spectrometry of lipid A and phospholipids from Acinetobacter baumannii cells

Delphine Vergoz
Annick Schaumann
Maria van Agthoven
Sara Martí
Jordi Vila
Stéphane Alexandre
  • Fonction : Auteur

Résumé

Acinetobacter baumannii, classified as priority number one by the World Health Organization (WHO), is an opportunistic pathogen responsible for infection and is able to develop antibiotic resistance easily. Membranes are bacteria's first line of defense against external aggression, such as antibiotics. A chemical modification of a lipid family or a change in lipid composition can lead to resistance to antibiotics. In this work, we analyzed different A. baumannii strains from various environments with different antibiotic resistance profiles, using matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT-ICR MS). This study shows that it is possible to describe the main lipidome (phospholipids and lipid A) from the simple preparation of lysed cells, and that despite the complexity of the mixture. This ultra-high resolution mass spectrometry technique enables the separation of isobaric ion, to report a new class of lipids. Given its performance, this technique can be used to quickly and reliably characterize the lipidome of clinical strains from different environments.

Domaines

Chimie
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Année Mois Jours
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jeudi 12 novembre 2026
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1 10 10
Année Mois Jours
Avant la publication
jeudi 12 novembre 2026
Fichier sous embargo
jeudi 12 novembre 2026
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Dates et versions

hal-04774266 , version 1 (12-11-2024)

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Delphine Vergoz, Annick Schaumann, Isabelle Schmitz, Maria van Agthoven, Sara Martí, et al.. Direct analysis by ultra-high-resolution mass spectrometry of lipid A and phospholipids from Acinetobacter baumannii cells. Biochimie, 2024, ⟨10.1016/j.biochi.2024.09.012⟩. ⟨hal-04774266⟩
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