Unveiling the cell biology of hippocampal neurons with dendritic axon origin - Archive ouverte HAL
Journal Articles Journal of Cell Biology Year : 2025

Unveiling the cell biology of hippocampal neurons with dendritic axon origin

Christopher Parperis

Abstract

In mammalian axon-carrying–dendrite (AcD) neurons, the axon emanates from a basal dendrite, instead of the soma, to create a privileged route for action potential generation at the axon initial segment (AIS). However, it is unclear how such unusual morphology is established and whether the structure and function of the AIS in AcD neurons are preserved. By using dissociated hippocampal cultures as a model, we show that the development of AcD morphology can occur prior to synaptogenesis and independently of the in vivo environment. A single precursor neurite first gives rise to the axon and then to the AcD. The AIS possesses a similar cytoskeletal architecture as the soma-derived AIS and similarly functions as a trafficking barrier to retain axon-specific molecular composition. However, it does not undergo homeostatic plasticity, contains lesser cisternal organelles, and receives fewer inhibitory inputs. Our findings reveal insights into AcD neuron biology and underscore AIS structural differences based on axon onset.
Fichier principal
Vignette du fichier
jcb_202403141.pdf (8.59 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive
Licence

Dates and versions

hal-04765935 , version 1 (04-11-2024)

Licence

Identifiers

Cite

Yuhao Han, Daniela Hacker, Bronte Catharina Donders, Christopher Parperis, Roland Thuenauer, et al.. Unveiling the cell biology of hippocampal neurons with dendritic axon origin. Journal of Cell Biology, 2025, 224 (1), ⟨10.1083/jcb.202403141⟩. ⟨hal-04765935⟩
0 View
0 Download

Altmetric

Share

More