Despite the political efforts, France is among the main consuming countries of pesticides in the world and the first at European level with a consumption of more than 85,900 tons/year (BNVD 1, 2019). The preservation of natural resources through the management of diffuse pollution related to pesticide use is considered as a major challenge in France and based on the identification of the highest risk areas. Our work aims to map the phytosanitary practices impact on human health and on natural resources, based on indicators of phytosanitary pressure (TFI: Treatment Frequency Index) and risk (IRSA: Indicator of Risk on the Applicator’s Health, and IRTE: Indicator of Toxicity Risk on the Environment). These indicators are calculated with the EToPhy2 software. This approach allows to present the spatial distribution of risk and phytosanitary pressure in the south-west of France (Gimone watershed). This mapping process is based on the scores of phytosanitary pressure and risk assigned to each plot, calculated from the applied dose of pesticides and their toxicity degree. The spatialization of the health and environmental impact of farmers’ phytosanitary practices enable us to identify the plots that represent the highest risk and their location to natural resources such as streams. The result could be used to improve the management of agricultural phytosanitary practices, taking into account the proximity of treated plots to different natural resources.