Deterministic lateral displacement for the sorting of extracellular vesicles from complex biological samples
Résumé
Extracellular Vesicles (EVs) are secreted by cells and found in biofluids, with concentration correlated with oncogenic signals, making them attractive biomarkers for liquid biopsy. The current gold-standard method to isolate EVs requires an ultracentrifugation (UC) step among others. The costs and complexity of this technique are forbiddingly high for many researchers, as for clinical implementation. This paper reports a microfluidic method for EVs isolation based on Deterministic Lateral Displacement (DLD) size sorting. We improved the design from our initial device and demonstrated the capacity to isolate EVs from cell culture media, with main population size distribution consistent with UC technique.
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