Article Dans Une Revue Autophagy Année : 2024

LC3B conjugation machinery promotes autophagy-independent HIV-1 entry in CD4 + T lymphocytes

La machinerie de conjugaison de LC3B favorise l'entrée du VIH-1 dans les lymphocytes T CD4+ indépendamment de l'autophagie

Résumé

HIV-1 entry into CD4 + T lymphocytes relies on the viral and cellular membranes' fusion, leading to viral capsid delivery in the target cell cytoplasm. Atg8/LC3B conjugation to lipids, process named Atg8ylation mainly studied in the context of macroautophagy/autophagy, occurs transiently in the early stages of HIV-1 replication in CD4 + T lymphocytes. Despite numerous studies investigating the HIV-1-autophagy interplays, the Atg8ylation impact in these early stages of infection remains unknown. Here we found that HIV-1 exposure leads to the rapid LC3B enrichment towards the target cell plasma membrane, in close proximity with the incoming viral particles. Furthermore, we demonstrated that Atg8ylation is a key event facilitating HIV-1 entry in target CD4 + T cells. Interestingly, this effect is independent of canonical autophagy as ATG13 silencing does not prevent HIV-1 entry. Together, our results provide an unconventional role of LC3B conjugation subverted by HIV-1 to achieve a critical step of its replication cycle.

Fichier principal
Vignette du fichier
Pradel et al R2.pdf (231.09 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-04741706 , version 1 (17-10-2024)

Licence

Identifiants

Citer

Baptiste Pradel, Guilhem Cantaloube, Marie Villares, Maika S Deffieu, Véronique Robert-Hebmann, et al.. LC3B conjugation machinery promotes autophagy-independent HIV-1 entry in CD4 + T lymphocytes. Autophagy, 2024, 20 (8), pp.1825-1836. ⟨10.1080/15548627.2024.2338573⟩. ⟨hal-04741706⟩
176 Consultations
273 Téléchargements

Altmetric

Partager

  • More