%0 Unpublished work
%T Inferring interphase chromosomal structure from multiplexed fluorescence in situ hybridization data: a unified picture from human and mouse cells
%+ Laboratoire Charles Coulomb (L2C)
%+ Université de Montpellier (UM)
%+ Catholic University of Leuven = Katholieke Universiteit Leuven (KU Leuven)
%A Remini, Loucif
%A Segers, Midas
%A Parmeggiani, Andrea
%A Carlon, Enrico
%8 2024-10-17
%D 2024
%Z Nonlinear Sciences [physics]
%Z Chemical Sciences/Polymers
%Z Physics [physics]
%Z Life Sciences [q-bio]/Genetics/Human geneticsPreprints, Working Papers, ...
%X <div><p>We analyze multiplexed fluorescence in situ hybridization (m-FISH) data for human and mouse cell lines. The m-FISH technique uses fluorescently-labeled single stranded probes which hybridize to specific chromosomal regions, thereby allowing the measurement of the spatial positions of up to ∼ 100 tagged sites for several thousands interphase chromosomes. Our analysis focuses on a wide range of different cell lines and two distinct organisms and provides a unified picture of chromatin structure for scales ranging from 5 kb (kilo bases) up to 2 Mb (mega bases), thus covering a genomic region of almost three orders of magnitude. Confirming recent analysis (Remini et al., Phys. Rev. E 109, 024408 ( 2024)) we show that there are two characteristic arrangements of chromatin referred to as phase α (crumpled globule) and β (looped domain) and discuss the physical properties of these phases. We show that a simple heterogeneous random walk model captures the main behavior observed in experiments and bring considerable insights on chromosomal structure.</p></div>
%G English
%2 https://hal.science/hal-04741167v1/document
%2 https://hal.science/hal-04741167v1/file/JCP_L.REMINI.pdf
%L hal-04741167
%U https://hal.science/hal-04741167
%~ CNRS
%~ L2C
%~ UNIV-MONTPELLIER
%~ ANR
%~ UM-2015-2021
%~ UM-EPE