Connecting physico-chemical characteristics with gelling properties of alginate like exopolymers recovered from wastewater granular sludge
Résumé
Alginate like exopolymers extracted from aerobic granules have shown interesting gelification properties. The composition of those extracts has been proven to be very complex, therefore the need to understand which molecules and mechanisms are responsible for the gelification properties is necessary. A fractionation method coupling anionic exchange chromatography with size exclusion chromatography has been used to separate molecules into fractions according to their global charge and size in order to determine which physico-chemical properties are the most important for their application as functional hydrogels. A miniaturized gelification screening method has been developed to compare the capacity of the collected fractions (corresponding to polymers with diversified charge and size) to gelify in standardized conditions.
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