The present article aims to explore the phenotypic variability of inflammatory reactions and compare them between two lines of goats. Hyper-selection based on the functional longevity of bucks successfully created two groups of goats with different lifespans (High_LGV and Low_LGV). Primiparous goats from the two divergent lines were injected intravenously with LPS (lipopolysaccharide) to stimulate inflammation. Blood was sampled at hours: -72, 0 (injection), 2, 4, 6, 8, 10, 12, 24, 48, 72, and 144. The concentration of 14 cytokines was measured: IFnγ, IL-1a, IL-1β, IL-4, IL-6, IL-10, IL-8, IL-17a, IL36RA, IP10, MIP1a, MIP1β, TNFa and VEGFA. The experiment was carried out in two experimental farms (n=83 goats). Results showed that the LPS injection induced a significant increase in several cytokines. The cytokines profiles were different between farms, highlighting different inflammatory backgrounds that could be related to different farm managements. The two longevity lines had different profiles for several cytokines within each farm. Most of the differences between lines were not consistent across farms, showing a potential GxE interaction. However, the increase of IL-6 was stronger among Low_LGV goats in both farms. These results strengthen our understanding of the immune reactions implied in animal resilience and longevity. This study has received funding from the European Union’s Horizon 2020 program SMARTER (agreement No 772787) and APIS-GENE (Paris, France).