Article Dans Une Revue Journal of Cell Biology Année : 2012

Microtubule binding by KNL-1 contributes to spindle checkpoint silencing at the kinetochore

Résumé

Accurate chromosome segregation requires coordination between microtubule attachment and spindle checkpoint signaling at the kinetochore. The kinetochore-localized KMN (KNL-1/Mis12 complex/Ndc80 complex) network, which mediates microtubule attachment and scaffolds checkpoint signaling, harbors two distinct microtubule-binding activities: the load-bearing activity of the Ndc80 complex and a less well-understood activity in KNL-1. In this paper, we show that KNL-1 microtubule-binding and -bundling activity resides in its extreme N terminus. Selective perturbation of KNL-1 microtubule binding in Caenorhabditis elegans embryos revealed that this activity is dispensable for both load-bearing attachment formation and checkpoint activation but plays a role in checkpoint silencing at the kinetochore. Perturbation of both microtubule binding and protein phosphatase 1 docking at the KNL-1 N terminus additively affected checkpoint silencing, indicating that, despite their proximity in KNL-1, these two activities make independent contributions. We propose that microtubule binding by KNL-1 functions in checkpoint silencing by sensing microtubules attached to kinetochores and relaying their presence to eliminate generation of the checkpoint signal.

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hal-04706709 , version 1 (24-09-2024)

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Julien Espeut, Dhanya K Cheerambathur, Lenno Krenning, Karen Oegema, Arshad Desai. Microtubule binding by KNL-1 contributes to spindle checkpoint silencing at the kinetochore. Journal of Cell Biology, 2012, 196 (4), pp.469-482. ⟨10.1083/jcb.201111107⟩. ⟨hal-04706709⟩
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