Detection of hemoplasmas and direct sequencing in blood from cattle reveals a diversity amongst Mycoplasma wenyonii species - Archive ouverte HAL
Poster De Conférence Année : 2024

Detection of hemoplasmas and direct sequencing in blood from cattle reveals a diversity amongst Mycoplasma wenyonii species

Résumé

Hemoplasmas are non-cultivable mycoplasmas that infect red blood cells of mammals. Several are found in cattle, such as Mycoplasma wenyonii (Mw) which is associated with chronic anemia, fever, edema and drop in milk production or ‘Candidatus M. haemobos’ (CMh) which impact on health is unclear. These infections are increasingly reported worldwide, yet their detection is mainly based on PCR of conserved genes like 16S and provides only partial insights into the diversity of circulating species and strains. Our inability to cultivate these bacteria limits genomic data availability for better identification, typing and understanding of their biology. In this study, we collected 583 blood samples from cattle across diverse farms and geographical regions in France. Using 16S qPCR, we detected hemoplasma DNA in 69% of samples. By employing whole-genome sequencing via Illumina and Oxford Nanopore Technologies on 6 highly positive samples, we successfully generated circular genomes for different strains. Comparative sequence analyses revealed the presence of at least two distinct Mw strains circulating within France. Subsequently, we developed a set of specific PCR probes to identify and differenciate Mw strains and CMh. Remarkably, Mw was detected in about 36% of the samples, while CMh was present in 55%, and co-infection by both species was observed in nearly 25% of cases. Interestingly, among Mw-positive animals, over 55% exhibited co-infection with multiple Mw strains. Our findings unraveled the co-circulation of CMh and diverse Mw strains in France. Notably, we report the novel observation of co-occurring Mw strains within the same animal for the first time. These results raised the question on the potential implication of specific strains, alone or in combination, in the developpement of clinical signs in cattle. This emphases the need of more comprehensive molecular epidemiology approaches for understanding hemoplasma infections.

Domaines

Bactériologie
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Dates et versions

hal-04696122 , version 1 (12-09-2024)

Identifiants

  • HAL Id : hal-04696122 , version 1

Citer

Chloé Saada, Hortensia Robert, Lucie Roques, Marie Claude Hygonenq, Renaud Maillard, et al.. Detection of hemoplasmas and direct sequencing in blood from cattle reveals a diversity amongst Mycoplasma wenyonii species. 25th Congres of the International Organization for Mycoplasmology, Jul 2024, Las Palmas De Gran Canaria, Spain. ⟨hal-04696122⟩
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