A transient radial cortical microtubule array primes cell division in Arabidopsis - Archive ouverte HAL
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2024

A transient radial cortical microtubule array primes cell division in Arabidopsis

Isaty Melogno
  • Fonction : Auteur
Shogo Takatani
  • Fonction : Auteur
Paula Llanos
  • Fonction : Auteur
Coralie Goncalves
  • Fonction : Auteur
Chie Kodera
  • Fonction : Auteur
Marjolaine Martin
  • Fonction : Auteur
Claire Lionnet
  • Fonction : Auteur
Magalie Uyttewaal
  • Fonction : Auteur
Martine Pastuglia
  • Fonction : Auteur
Christophe Trehin
  • Fonction : Auteur
David Bouchez
  • Fonction : Auteur
Jacques Dumais
  • Fonction : Auteur
Olivier Hamant

Résumé

Because all cells experience mechanical stress, they have to develop resistance mechanisms to survive. In plants, this notably involves guidance of cellulose deposition by cortical microtubules in the direction of maximal tensile stress. Although the formation of new walls during cell division tends to follow maximal tensile stress direction too, analyses of individual cells over time reveal a much more variable behavior. The origin of such variability, as well as the exact role of interphasic microtubule behavior before cell division have remained mysterious so far. To approach this question, we took advantage of the Arabidopsis stem, where the tensile stress pattern is both highly anisotropic and stable. Although cortical microtubules generally align with maximal tensile stress, we detected a specific time window, ca. 3 hours before cell division, where cells form a radial pattern of cortical microtubules. This pattern was observed in different growth conditions, and was not related to cell geometry or polar auxin transport. Interestingly, this cortical radial pattern correlated with the well-documented increase of cytoplasmic microtubule accumulation before cell division. This radial organization was prolonged in cells of the trm678 mutant, where cortical microtubules are partially disorganized.

Whereas division plane orientation in trm678 is noisier, we found that cell division symmetry was in contrast more precise. We propose that an increased cytoplasmic microtubule accumulation in late G2 disrupts cortical microtubules alignment with tissue stress, allowing the cell to transiently explore its own geometry in order to select a future division plane with correct orientation and symmetry.

SIGNIFICANCE STATEMENT

In all kingdoms, cells divide according to their own geometry as well as external cues. We discovered a transient stage in plant cells, where part of the division machinery becomes blind to mechanical forces originating from the tissue. Using quantitative imaging and mutant analysis, we propose that this new pre-mitotic stage allows cells to take their own geometry into account and increase the precision of the following division.

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Dates et versions

hal-04691139 , version 1 (07-09-2024)

Identifiants

Citer

Isaty Melogno, Shogo Takatani, Paula Llanos, Coralie Goncalves, Chie Kodera, et al.. A transient radial cortical microtubule array primes cell division in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 2024, 121 (29), pp.e2320470121. ⟨10.1073/pnas.2320470121⟩. ⟨hal-04691139⟩
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