Article Dans Une Revue Advances in Experimental Medicine and Biology Année : 2024

The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions

Résumé

Nuclear magnetic resonance (NMR) and native mass spectrometry (MS) are mature physicochemical techniques with long histories and important applications. NMR spectroscopy provides detailed information about the structure, dynamics, interactions, and chemical environment of biomolecules. MS is an effective approach for determining the mass of biomolecules with high accuracy, sensitivity, and speed. The two techniques offer unique advantages and provide solid tools for structural biology. In the present review, we discuss their individual merits in the context of their applications to structural studies in biology with specific focus on protein interactions and evaluate their limitations. We provide specific examples in which these techniques can complement each other, providing new information on the same scientific case. We discuss how the field may develop and what challenges are expected in the future. Overall, the combination of NMR and MS plays an increasingly important role in integrative structural biology, assisting scientists in deciphering the three-dimensional structure of composite macromolecular assemblies.

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Dates et versions

hal-04688936 , version 1 (05-09-2024)

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Citer

Elisabetta Boeri Erba, Annalisa Pastore. The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions. Advances in Experimental Medicine and Biology, 2024, Advanced Technologies for Protein Complex Production and Characterization, 1453, pp.109-123. ⟨10.1007/978-3-031-52193-5_8⟩. ⟨hal-04688936⟩
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