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Pré-Publication, Document De Travail Année : 2024

More than a Mutagenic Aflatoxin B1 Precursor: The Multiple Cellular Targets of Versicolorin a Revealed by Global Gene Expression Analysis

Résumé

Versicolorin A (VerA), a precursor of the potent carcinogen aflatoxin B1 (AFB1), is an emerging mycotoxin. Recent research has highlighted the mutagenic and genotoxic properties of VerA, yet several facets of its pronounced toxicity remain unexplored. In the present study, we investigated early (6 h) transcriptomic changes induced by VerA in differentiated intestinal cells in non-cytotoxic conditions (1 and 3 µM) and compared its effects to those of AFB1 at 1 µM. Our findings indicated that VerA led to substantial alterations in global gene expression profiles, while AFB1 did not exhibit the same effect. As expected, both toxins caused alterations in gene expression associated with well-known aspects of their toxicity, including mutagenicity, genotoxicity, oxidative stress, and apoptosis. However, we also observed novel features of VerA toxicity, including the ability to cause mitochondrial dysfunction and to trigger a type-1 interferon response at least partially mediated by cGAS-STING. VerA also induced changes in the expression of genes involved in the regulation of cell shape and adhesion, transcription/translation as well as genes associated with tumor biology. Our results offer new evidence of the high toxicity of VerA and underscore the importance of further assessing the risk associated with its presence in food.

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Dates et versions

hal-04687397 , version 1 (04-09-2024)

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Carine Al-Ayoubi, Ophelie Rocher, Claire Nayles, Yannick Lippi, Julien Vignard, et al.. More than a Mutagenic Aflatoxin B1 Precursor: The Multiple Cellular Targets of Versicolorin a Revealed by Global Gene Expression Analysis. 2024. ⟨hal-04687397⟩
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