Articular cartilage lacks the ability to repair itself because it is devoid of blood vessels, nerves, and lymph, and there is no effective treatment that contributes to the cartilage repair process. Osteoarthritis (OA) represents a difficult challenge and an increasing health care burden worldwide. Nowadays, the generation of scaffolds has received great and increasing attention in order to preserve the biophysical environment and bioactive factors. In addition, improved decellularization technology has contributed to providing new promising solutions in the treatment of OA.