Communication Dans Un Congrès Année : 2021

Charged Particle Irradiation Effects on Human Chondrosarcoma Cells

Résumé

Chondrosarcoma is well known as a radioresistant tumor. The treatment with charged particle (hadron therapy) has shown better speci city and lower toxicity for the surrounding normal tissue when compared to conventional photon therapy. The present study focuses on evaluating cellular e ects of proton irradiation on directly exposed chondrosarcoma cells and bystander normal cells. Chondrosarcoma tumor cells (SW1353 and L835) were directly exposed to a low energy ( ~20 MeV) proton beam line at doses ranging from 0.1 to 4Gy. To study the bystander phenomena we used a medium transfer protocol from the tumor cells irradiated with 0-2Gy to normal cells (T/C-28a2 chondrocyte and EA.hy926 endothelial cell lines). Cell survival, cell death and DNA damage were quanti ed in directly irradiated chondrosarcoma cells and in bystander chondrocytes and endothelial cells. Proton irradiation induces a dose dependent direct e ect in chondrosarcoma cells as augmented number of micronuclei at 24 h after exposure, a reduction of the long-term cellular survival, an elevation in caspase activity at 24h and a decreased metabolic activity after 5 days. In bystander chondrocyte cells, SW1353 cell line induced an increase in micronuclei and a decrease in long term survival at 0.1 Gy, while L835 cells showed an increase in micronuclei number for all doses. In contrast, chondrosarcoma L835 cells induce both an elevation of micronuclei number and a decrease in long-term survival at the dose of 0.5 Gy in endothelial cells, while SW1353 chondrosarcoma cells induce both an increase in micronuclei number and a decrease in survival at 2Gy. The outcomes showed that proton irradiated chondrosarcoma cells release stress factors inducing a wide bystander response in the non-irradiated chondrocyte/endothelial cells.

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Dates et versions

hal-04674898 , version 1 (21-08-2024)

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  • HAL Id : hal-04674898 , version 1

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Mihaela Tudor, François Chevalier, Diana Savu. Charged Particle Irradiation Effects on Human Chondrosarcoma Cells. 46th Annual Meeting of the European Radiation Research Society - ERRS 2021, Nov 2021, Caen, France. ⟨hal-04674898⟩
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