Poster De Conférence Année : 2020

Bystander effects induced by proton-irradiated human chondrosarcoma cells

Résumé

The radiation-induced bystander effect was highlighted in non-irradiated cells that received stress signals sent by the irradiated neighbour cells. The bystander effect depends on a number of conditions such as radiation dose, dose rate, LET, cell type, culture system and its contribution is appraised for conventional therapy as well as charged particle therapy (hadron therapy). Hadron therapy presents better specificity and lower toxicity for the surrounding normal tissue compared to conventional treatment and is standard care for some chondrosarcomas. This study focuses on analysing cellular effects induced in bystander cells by 2D and 3D chondrosarcoma cells irradiated with protons of high and low energy versus X-rays. Bystander effects were induced through media transferred from 2D and 3D irradiated chondrosarcoma cells (SW1353) to normal chondrocytes (T/C-28a2) and endothelial cells (EA.hy926). Cell survival and DNA damage were assessed in bystander cells. Irradiated 2D chondrosarcoma cells triggered bystander effects in both chondrocytes and endothelial cells. Interestingly, X-Ray irradiated 2D chondrosarcoma cells caused a more efficient bystander effects in chondrocytes than proton irradiated cells at the lowest dose used (0.1 Gy). On the contrary, in endothelial cells the most effective bystander response was caused by the low energy proton irradiation at 2 Gy. Proton irradiated 3D spheroids induced a similar response in 2D bystander cells. Our results showed the ability of chondrosarcoma cells to secrete bystander signals that were received by normal chondrocyte and endothelial cells.

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Dates et versions

hal-04674882 , version 1 (21-08-2024)

Identifiants

  • HAL Id : hal-04674882 , version 1

Citer

Mihaela Tudor, Mihaela Temelie, Roxana Cristina Popescu, Anna Rzjanina, Gennady Mytsin, et al.. Bystander effects induced by proton-irradiated human chondrosarcoma cells. Eighth International Conference on Radiation in Various Fields of Research - RAD 2020 virtual Conference, Jun 2020, Herceg Novi, Montenegro. . ⟨hal-04674882⟩
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