Chapitre D'ouvrage Année : 2014

Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins

Résumé

Membrane proteins (MPs) play key roles in most important cellular processes ranging from cell-to-cell communication to signaling processes. Despite recent improvements, the expression of functionally folded MPs in sufficient amounts for functional and structural characterization remains a challenge. Indeed, it is still difficult to predict whether a protein can be overproduced in a functional state in some expression system(s), although studies of high-throughput screens have been issued in recent years. Prokaryotic expression systems present several advantages over eukaryotic ones. Among them, Lactococcus lactis (L. lactis) has emerged in the past years as a good alternative expression system to Escherichia coli. The purpose of this chapter is to describe L. lactis and its tightly inducible system, nisin-controlled gene expression (NICE), for the expression of MPs from both prokaryotic and eukaryotic origins. Moreover, we will describe the functional characterization of some MPs produced in L. lactis.

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Dates et versions

hal-04674463 , version 1 (21-08-2024)

Identifiants

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Sana Bakari, François André, Daphnée Seigneurin-Berny, Marcel Delaforge, Norbert Rolland, et al.. Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins. Mus-Veteau, I. (eds). Membrane Proteins Production for Structural Analysis, Springer, New York, NY, pp.107-132, 2014, 978-1-4939-0662-8. ⟨10.1007/978-1-4939-0662-8_5⟩. ⟨hal-04674463⟩
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