In vitro and in vivo evaluation of thapsigargin as an antiviral agent against transmissible gastroenteritis virus - Archive ouverte HAL
Article Dans Une Revue Veterinary Research Année : 2024

In vitro and in vivo evaluation of thapsigargin as an antiviral agent against transmissible gastroenteritis virus

Yang Li
  • Fonction : Auteur
Yuanyuan Liu
  • Fonction : Auteur
Yunhang Zhang
  • Fonction : Auteur
Chen Tan
  • Fonction : Auteur
Yifei Cai
  • Fonction : Auteur
Yue Zhang
  • Fonction : Auteur
Jianing Chen
  • Fonction : Auteur
Yuguang Fu
  • Fonction : Auteur
Guangliang Liu
Connectez-vous pour contacter l'auteur

Résumé

AbstractSwine enteric coronaviruses (SeCoVs) pose a significant threat to the global pig industry, but no effective drugs are available for treatment. Previous research has demonstrated that thapsigargin (TG), an ER stress inducer, has broad-spectrum antiviral effects on human coronaviruses. In this study, we investigated the impact of TG on transmissible gastroenteritis virus (TGEV) infection using cell lines, porcine intestinal organoid models, and piglets. The results showed that TG effectively inhibited TGEV replication both in vitro and ex vivo. Furthermore, animal experiments demonstrated that oral administration of TG inhibited TGEV infection in neonatal piglets and relieved TGEV-associated tissue injury. Transcriptome analyses revealed that TG improved the expression of the ER-associated protein degradation (ERAD) component and influenced the biological processes related to secretion, nutrient responses, and epithelial cell differentiation in the intestinal epithelium. Collectively, these results suggest that TG is a potential novel oral antiviral drug for the clinical treatment of TGEV infection, even for infections caused by other SeCoVs.
Fichier principal
Vignette du fichier
13567_2024_Article_1359.pdf (4.34 Mo) Télécharger le fichier
Origine Publication financée par une institution

Dates et versions

hal-04667533 , version 1 (05-08-2024)

Identifiants

Citer

Yang Li, Yuanyuan Liu, Yunhang Zhang, Chen Tan, Yifei Cai, et al.. In vitro and in vivo evaluation of thapsigargin as an antiviral agent against transmissible gastroenteritis virus. Veterinary Research, 2024, 55 (1), pp.97. ⟨10.1186/s13567-024-01359-x⟩. ⟨hal-04667533⟩

Collections

ARINRAE-VR ARINRAE
35 Consultations
5 Téléchargements

Altmetric

Partager

More