New Antifungal Susceptibility Test Based on Chitin Detection by Image Cytometry - Archive ouverte HAL
Article Dans Une Revue Antimicrobial Agents and Chemotherapy Année : 2019

New Antifungal Susceptibility Test Based on Chitin Detection by Image Cytometry

Yan Wang
  • Fonction : Auteur
Arisandy Andriampamonjy
  • Fonction : Auteur
Cécile Garnaud
  • Fonction : Auteur
Danièle Maubon
  • Fonction : Auteur
Muriel Cornet
Delphine Aldebert

Résumé

The antifungal susceptibility tests used in clinical laboratories have several limitations. We developed a new test, SensiFONG, based on the detection of chitin levels after exposure to antifungal drugs. The optimal culture conditions were 30°C for 6 h for yeast strains and 26°C for 16 h for molds. The strains were exposed to a range of echinocandin or azole concentrations. Chitin was stained with calcofluor white.

Dates et versions

hal-04661471 , version 1 (24-07-2024)

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Citer

Yan Wang, Arisandy Andriampamonjy, Sebastien Bailly, Cécile Garnaud, Danièle Maubon, et al.. New Antifungal Susceptibility Test Based on Chitin Detection by Image Cytometry. Antimicrobial Agents and Chemotherapy, 2019, 64 (1), ⟨10.1128/AAC.01101-19⟩. ⟨hal-04661471⟩

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