1 H and 31 P-NMR Assignments of the Non-exchangeable Protons of the Consensus Acceptor Exon: Intron Junction d(CpTpApCpApGpGpT) - Archive ouverte HAL
Article Dans Une Revue Journal of Biomolecular Structure and Dynamics Année : 1986

1 H and 31 P-NMR Assignments of the Non-exchangeable Protons of the Consensus Acceptor Exon: Intron Junction d(CpTpApCpApGpGpT)

Résumé

The consensus acceptor exon:intron junction d(CpTpApCpApGpGpT) has been synthesized by a modified phosphotriester method. The non-self complementary octamer exists in the single strand form in aqueous buffer at 20 degrees C as evidenced by temperature variable 1H-NMR and NOE measurements. The non-exchangeable proton assignments were secured using a combination of techniques including two-dimensional COSY, NOESY and the double quantum technique 1H-1H-INADEQUATE as well as inversion recovery T1 experiments. The new technique of 31P-1H shift correlation is particularly valuable in removing certain ambiguities in the sugar proton assignments. Characteristic chemical shifts for the base protons which are determined by their immediate molecular environments are also useful in assignments. The consensus acceptor exon:intron junction adopts a random coil conformation in solution under the experimental conditions employed.

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Chimie
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Dates et versions

hal-04652404 , version 1 (18-07-2024)

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J. William Lown, Ding-Kwo Chang, Francoise Debart, Bernard Rayner, Jean-Louis Imbach. 1 H and 31 P-NMR Assignments of the Non-exchangeable Protons of the Consensus Acceptor Exon: Intron Junction d(CpTpApCpApGpGpT). Journal of Biomolecular Structure and Dynamics, 1986, 3 (6), pp.1171-1187. ⟨10.1080/07391102.1986.10508493⟩. ⟨hal-04652404⟩

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