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Article Dans Une Revue Angewandte Chemie International Edition Année : 2024

Lanthanide‐Based Probes for Imaging Detection of Enzyme Activities by NIR Luminescence, T1‐ and ParaCEST MRI

Résumé

Abstract Applying a single molecular probe to monitor enzymatic activities in multiple, complementary imaging modalities is highly desirable to ascertain detection and to avoid the complexity associated with the use of agents of different chemical entities. We demonstrate here the versatility of lanthanide (Ln 3+ ) complexes with respect to their optical and magnetic properties and their potential for enzymatic detection in NIR luminescence, CEST and T1 MR imaging, controlled by the nature of the Ln 3+ ion, while using a unique chelator. Based on X‐ray structural, photophysical, and solution NMR investigations of a family of Ln 3+ DO3A‐pyridine model complexes, we could rationalize the luminescence (Eu 3+ , Yb 3+ ), CEST (Yb 3+ ) and relaxation (Gd 3+ ) properties and their variations between carbamate and amine derivatives. This allowed the design of probes which undergo enzyme‐mediated changes detectable in NIR luminescence, CEST and T1‐weighted MRI, respectively governed by variations in their absorption energy, in their exchanging proton pool and in their size, thus relaxation efficacy. We demonstrate that these properties can be exploited for the visualization of β‐galactosidase activity in phantom samples by different imaging modalities: NIR optical imaging, CEST and T1‐weighted MRI.

Dates et versions

hal-04634298 , version 1 (03-07-2024)

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Rémy Jouclas, Sophie Laine, Svetlana Eliseeva, Jérémie Mandel, Frédéric Szeremeta, et al.. Lanthanide‐Based Probes for Imaging Detection of Enzyme Activities by NIR Luminescence, T1‐ and ParaCEST MRI. Angewandte Chemie International Edition, 2024, 63 (16), pp.e202317728. ⟨10.1002/anie.202317728⟩. ⟨hal-04634298⟩
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