BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa - Archive ouverte HAL
Journal Articles Frontiers in Microbiology Year : 2024

BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa

Abstract

Previously, we pointed out in P. aeruginosa PAO1 biofilm cells the accumulation of a hypothetical protein named PA3731 and showed that the deletion of the corresponding gene impacted its biofilm formation capacity. PA3731 belongs to a cluster of 4 genes ( pa3732 to pa3729 ) that we named bac for “Biofilm Associated Cluster.” The present study focuses on the PA14_16140 protein, i.e., the PA3732 (BacA) homolog in the PA14 strain. The role of BacA in rhamnolipid secretion, biofilm formation and virulence, was confirmed by phenotypic experiments with a bacA mutant. Additional investigations allow to advance that the bac system involves in fact 6 genes organized in operon, i.e., bacA to bacF. At a molecular level, quantitative proteomic studies revealed an accumulation of the BAC cognate partners by the bacA sessile mutant, suggesting a negative control of BacA toward the bac operon. Finally, a first crystallographic structure of BacA was obtained revealing a structure homologous to chaperones or/and regulatory proteins.

Dates and versions

hal-04630144 , version 1 (01-07-2024)

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Lisa Wallart, Mohamed Amine Ben Mlouka, Brahim Saffiedine, Laurent Coquet, Hung Le, et al.. BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa. Frontiers in Microbiology, 2024, 15, pp.1332448. ⟨10.3389/fmicb.2024.1332448⟩. ⟨hal-04630144⟩
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