Deep phenotyping characterization of human unconventional CD8+NKG2A/C+ T cells among T and NK cells by spectral flow cytometry

Here, we present a protocol for setting three spectral flow cytometry panels for the characterization of human unconventional CD8+NKG2A/C+ T cells as well as other T and natural killer cell subsets. We describe steps for standardizing, preparing, and staining the cells, the experimental setup, and the final data analysis. This protocol should be advantageous in various settings including immunophenotyping of limited samples, immune function evaluation/monitoring, as well as research in oncology, autoimmune, and infectious diseases.

Mots clés

Flow Cytometry Immunology Antibody

Domaines

Sciences du Vivant [q-bio]

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1-s2.0-S2666166723007013-main.pdf (8.19 Mo)

Origine	Publication financée par une institution
Licence	Paternité - Pas d'utilisation commerciale - Pas de modification

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https://hal.science/hal-04613624

Soumis le : mardi 10 septembre 2024-16:10:05

Dernière modification le : vendredi 11 octobre 2024-10:18:03

Dates et versions

hal-04613624 , version 1 (10-09-2024)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

HAL Id : hal-04613624 , version 1
DOI : 10.1016/j.xpro.2023.102734
PUBMED : 38032799
PUBMEDCENTRAL : PMC10711235

Citer

Aurelio Orta-Resendiz, Caroline Petitdemange, Sandrine Schmutz, Béatrice Jacquelin, Sophie Novault, et al.. Deep phenotyping characterization of human unconventional CD8+NKG2A/C+ T cells among T and NK cells by spectral flow cytometry. STAR Protocols, 2023, 4 (4), pp.102734. ⟨10.1016/j.xpro.2023.102734⟩. ⟨hal-04613624⟩

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