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Article Dans Une Revue Journal of Chromatography A Année : 2024

Investigating cationic and zwitterionic successive multiple ionicpolymer layer coatings for protein separation by capillary electrophoresis

Henry Frick
  • Fonction : Auteur
Alisa Höchsmann
Norbert Schaschke
  • Fonction : Auteur
Christian Neusüß

Résumé

Successive multiple ionic-polymer layers (SMILs) have long since proved their worth in capillary electrophoresis as they ensure stable electroosmotic flow (EOF) and relatively high separation efficiency. Recently, we demonstrated that plotting the plate height (H) against the solute migration velocity (u) enabled a reliable quantitative evaluation of the coating performances in terms of separation efficiency. In this work, various physicochemical and chemical parameters of the SMIL coating were studied and optimized in order to decrease the slope of the ascending part of the H vs u curve, which is known to be controlled by the homogeneity in charge of the coating surface and by the possible residual solute adsorption onto the coating surface. SMILs based on poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium styrene sulfonate) (PSS) were formed and the effect of each polyelectrolyte molar mass and of the number of polyelectrolyte layers (up to 21 layers) was studied. The use of polyethylene imine as an anchoring first layer was considered. More polyelectrolyte couples based on PDADMAC, polybrene, PSS, poly(vinyl sulfate), and poly(acrylic acid) were tested. Finally, zwitterionic polymers based on the poly(α-l-lysine) scaffold were synthesized and used as the last layer of SMILs, illustrating their ability to finetune the EOF, while maintaining good separation efficiency.
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Dates et versions

hal-04605815 , version 1 (08-06-2024)

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Laura Dhellemmes, Laurent Leclercq, Henry Frick, Alisa Höchsmann, Norbert Schaschke, et al.. Investigating cationic and zwitterionic successive multiple ionicpolymer layer coatings for protein separation by capillary electrophoresis. Journal of Chromatography A, 2024, 1720, pp.464802. ⟨10.1016/j.chroma.2024.464802⟩. ⟨hal-04605815⟩
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